毛细管电泳紫外检测苦荞中的D-手性肌醇

Determination of D-chiro-inostiol in buckwheat by high performance capillary electrophoresis with indirect ultraviolet detection

采用高效毛细管电泳-紫外检测法,对苦荞籽粒中D-手性肌醇进行检测。在100 mmol/L的硼砂缓冲液,分离电压10 kV,检测波长195 nm条件下进行紫外检测。结果表明:标准曲线线性范围在0.1~150 mg/mL之间(R^2>0.999),检出限均低于0.02 mg/mL,加标回收率为96%~108%,相对标准偏差4.3%。该方法简便快捷、准确可靠,而且采用水溶液作为流动相,可为企业大量测定苦荞样品中D-手性肌醇降低检测成本,提高检测效率,减少环境污染。

D-chiro-inostiol in buckwheat was determined by high performance capillary electrophoresis （HPCE） with ultraviolet detector, with100 mmoL/L borate buffer, voltage 10kV and detection wavelength 195 nm. The results showed that the linear range was 0.1 - 150 mg/mL with correlation coefficients more than 0. 999 and the detection limit less than 0.02 mg/mL （Rs/N = 3）. The average recoveries were in the range of 9% -108%, with the relative standard deviation （RSD） 4.3%. This method is convenient, accurate and reliable, which can decrease the cost and pollution, increase the efficiency with aqueous so- lution as moving phase.