羊栖菜多糖提取物及其润肤霜的保湿和抗氧化活性

Moisturizing and antioxidant activity of polysaccharide extract from Sargassum fusiforme and its application in moisturizing cream

采用超声细胞破碎辅助水提-醇沉法提取羊栖菜多糖(FSF),测定其抗氧化活性和保湿性能,并用其制备润肤霜,体外评价该润肤霜的保湿性能,使用皮肤外涂实验考察该润肤霜对小鼠皮肤抗氧化活性的影响。结果表明:羊栖菜多糖提取物(EFSF)具有较强的自由基清除能力,保湿性能优于甘油;该润肤霜同样具有较好的保湿性能,在干燥环境下198 h的失水率为4.33%,在相对湿度43%环境下198 h的失水率为2.36%;皮肤外涂实验表明,与阴性对照组(仅含基质的润肤霜)、阳性对照组(0.3%熊果苷润肤霜)相比,该润肤霜均可显著增强小鼠皮肤的羟自由基清除能力(403.65 U/mgprot)、超氧阴离子自由基清除能力(810.52 U/gprot)、总抗氧化能力(11.58 U/mgprot)和T-SOD活性(81.11 U/mgprot),显著提高羟脯氨酸含量(43.49 mg/L)以及降低MDA含量(2.12 nmol/mgprot)。

The sargassum polysaccharides （FSF） was extracted from Sargassum fusiforme by using ultrasonic cell disintegration - assisted water extraction and alcohol precipitation. Determination of antioxidant activity and moisturizing activity of the extract of FSF （EFSF） was carried out. This EFSF was used to prepare moisturizing cream, and its moisturizing activity was evaluated in vitro. The effect of the moisturizing cream on the antioxidant activity of the skin of the mouse was investigated by using the skin coating test. Results show that EFSF has strong free radical scavenging ability and better moisturizing activity than glycerol. The cream with EFSF also has high moisturizing activity：the water loss rate of 198 h in a dry environment is 4. 33%, the water loss rate of 198 h under the relative humidity of 43% is 2. 36%. Skin coating experiments show that compared with the negative control group （cream contained only substrate） and with the positive control group （0. 3 % arbutin moisturizing cream）, this moisturizing cream with EFSF can significantly enhance the hydroxyl radical scavenging capacity （403.65 U/mgprot）, superoxide anion radical scavenging ability （810. 52 U/gprot）, total antioxidant capacity （ 11.58 U/mgprot）, and T - SOD activity （ 81.11 U/mgprot） in mouse skin, markedly increase hydroxyproline content （43.49 mg/L） and reduce MDA content （2. 12 nmol/mgprot）.