高产红曲色素和Monacolin K红曲霉菌的诱变选育

Mutation Breeding of High-yield Monascus Pigments and Monacolin K

采用紫外-氯化锂复合诱变方法筛选高产红曲色素及Monacolin K的红曲霉菌株,最佳诱变条件为紫外诱变时长2min,氯化锂浓度0.6‰。对突变株菌落形态进行初筛,对红曲色素和Monacolin K含量检测进行复筛,并进行遗传稳定性实验,得到了高产红曲色素、Monacolin K且遗传稳定性能好的突变菌株QH12,其红曲色素色价达到2450U/g,Monacolin K产量达1.68mg/g,分别是原始菌株的1.84倍和4.67倍,相对于原始菌株有较大提高。

Monascus is mutated by ultraviolet high-yield Monascus pigments and Monacolin K. radiation and lithium chloride to obtain strains with The best mutagenic conditions are found that ultraviolet mutation duration of 2 min, and lithium chloride concentration of 0. 6%0. After screening colonial morphology, test the color value of Monascus pigments and the concentration of Monacolin K, and assess the genetic stability, the mutant strain QH12 with a high-yield Monasctts pigments and Monacolin K is obtained. As for QH12, the color value of Monascus pigments is 2450 U/g, and the concentration of Monacolin K is 1.68 mg/g, 1.84 times and 4.67 times respectively as much as those of the original strain.