山羊胎肝内定位注射不同体积间充质干细胞对胎羊发育的影响

Effect of stereotaxic injection of mesenchymal stem cells of different volumes into goat fetal liver on the development of fetal goat

目的探索山羊胎肝内定位注射不同体积间充质干细胞(MSC)对胎羊发育的影响。方法以B超探查10只疑似怀孕的母山羊,观察胎羊数量、胎羊性别,确定胎肝位置。怀孕约3个月时,在B超引导下穿刺至胎肝,注射不同体积和不同数量的雄性山羊骨髓MSC悬液到胎肝实质区域,精心护理并观察穿刺母羊是否流产。结果借助B超探查10只疑似怀孕母羊中的6只怀孕,可以发现胎心跳动和胎肝血流并确定胎肝的位置,在B超引导下能够准确注射MSC悬液至胎肝实质区域,注射1 ml MSC悬液,不会引起流产,安全可行;而注射同等细胞数量的4 ml MSC悬液,则导致穿刺山羊流产。结论 B超显影可确诊山羊怀孕和确定胎肝位置,胎肝内注射1 m山羊骨髓MSC悬液不会导致胎羊流产,MSC数量不变而体积增加至4 ml时易导致胎羊流产。

Objective To explore the effect of stereotaxic injection of mesenchymal stem cells of different volumes into goat fetal liver on the development of fetal goat. Methods Ten suspected pregnant she-goats received B-mode ultrasonography, the number and gender of fetal goats were observed, and the position of fetal liver was determined. About three months of pregnancy, these she-goats received B-mode uhrasonography guided puncture to the fetal liver and injected with the suspension of caprine bone marrow-derived MSCs in different volumes and with different numbers of cells into the parenchyma region of fetal liver. They were then carefully nursed and observed whether they would have abortion or not. lleaulta Through B-mode ultrasonography, six of 10 suspected pregnant she-goats were diagnosed pregnant, with the fetal heat found beating and the fetal liver blood flowing, and the position of fetal liver was determined; guided by the B-mode ultrasonography, the MSC suspension could be accurately injected into the parenchyma region of fetal liver; injection of lml MSC suspension was safe and （lid not lead to abortion, while injection of 4ml MSC suspension with equal number of cells resulted in abortion. Conclusion B-mode ultrasonography could help diagnose pregnancy of she-goats and determine the position of fetal liver; injection of l ml suspension of caprine bone nmrrow-derived MSCs into the fetal liver will not lead to abortion, while injection of 4ml suspension with equal number of MSCs is prone to cause abortion.