慢性应激对小鼠胃肠运动及血清GAS、MTL的影响

Effects of Chronic Stress on Gastrointestinal Motility,Serum Levels of GAS and MTL of Mice

目的:研究不可预知的慢性应激对小鼠胃肠运动及血清胃动素(GAS)、胃动素(MTL)的影响,以期验证慢性应激可以导致肝郁脾虚型功能性消化不良(FD)小鼠模型。方法:将40只小鼠随机分为正常组和模型组各20只,采用食物剥夺、明暗颠倒及夹尾束缚等9种方法造模,分别观察小鼠的饮水量、进食量及体重改变情况,造模21天后测定血清中胃动素(GAS)、胃动素(MTL)含量,并取胃窦及十二指肠组织进行病理检查;胃肠排空功能测定。结果:不可预知的慢性应激可以使FD模型小鼠的进食下降、体重增加放缓,小肠推进率下降,胃残留率增加;同时出现血清GAS、MTL含量降低,和正常组比较,差异具有显著性(P<0.01),而胃、十二指肠组织的病理表现无明显改变。结论:不可预知的慢性应激能够成功导致肝郁脾虚型FD小鼠模型。

Objective： To study the effects of unpredictable chronic stress on gastrointestinal movement, serum gastric dynamic element （GAS）, the moving element （MTL） of mice in order to verify that chronic stress can lead to the mouse model of functional dyspepsia （FD） caused by liver depression and spleen deficiency. Method： 40 mice were randomly and evenly divided into a normal group and a model group. 9 methods, such as food deprivation, light and shade reversing and tail restraint, were adopted to mold, and water intake, food intake and weight change of mice were observed. After 21 days＇ of modeling, the contents of the serum （GAS） and gastric motilin （MTL） were determined, and the pathological examinations of the gastric sinus and duodenal tissues were performed. Gastrointestinal emptying function was determined. Result： Unpredictable chronic stress reduced FD model mice＇ s appetite, slowed down their weight gain, decreased their small intestinal propulsive rate, and increased their stomach＇ s residue rate. Compared with the normal group, their serum MTL and GAS levels decreased, which had significant difference （P〈0.01） , while there were no obvious pathological changes of the stomach and duodenum. Conclusion ： The unpredictable chronic stress can lead to the success of the liver and spleen deficiency FD mice models. Key words： functional dyspepsia ; gastrointestinal motility ; gastrin ; motilin