酶解辅助乙醇提取牡丹果皮木犀草素的研究

Enzyme assisted ethanol extraction of luteolin from Paeonias suffruticosa pericarp

以"凤丹"牡丹(Paeonias suffruticosa"Feng Dan")果皮为原料,采用酶解辅助乙醇提取牡丹果皮木犀草素,以木犀草素得率为评价指标,先以乙醇体积分数、浸泡时间、液料比、提取次数为考察因素,在单因素实验的基础上,利用正交实验法得出四个因素的最优组合。在此最优组合的基础上,再以酶用量、水浴时间、p H、水浴温度为考察因素,利用正交实验法得到酶解条件的最优组合。结果显示:在乙醇体积分数为60%、果皮粉末浸泡30 min、液料比为10∶1条件下提取三次,以果胶酶0.05 g·L^(-1)、水浴加热160 min、p H4.0、水浴温度55℃条件下预处理的牡丹果皮木犀草素得率最高达19.30 mg·g^(-1)。

Using the method of enzyme assisted ethanol extraction of luteolin in Paeonias suffruticosa ＂Feng Dan＂ pericarp. Firstly,taking the yield of luteolin as assessment index, effects of volume fraction of ethanol, soak time,liquid to solid ratio,the extraction times were investigated,and the optimal combination was discovered by orthogonal experiment on the basis of single factor experiment.On this basis, effects of pectinase amount, water bath time, pH, water bath temperature were investigated, and the optimal combination was discovered by orthogonal experiment on the basis of single factor experiment.The optimal factors conbination was as follows：the pericarp pretreated with pectinase content of 0.05g·L^-1,water bath time of 160 minutes, pH4.0,water bath temperature of 55 ℃, and then extracted in 60% ethanol for 30 min,liquid to solid ratio of 10：1 ,extract for 3 times, and the maximum yield of luteolin was up to 19.30 mg·g^-1.