双孢菇废弃菇柄多糖的超声波提取及其体外抗氧化活性

Optimization of ultrasonic extraction and in vitro antioxidant activity of polysaccharide from Agaricus bisporus stipe

为了提高双孢菇菇柄的综合利用率,以双孢菇废弃菇柄为原料,通过单因素实验探讨了超声功率、超声提取时间、液固比、超声提取次数、醇沉体积对双孢菇菇柄多糖得率的影响,采用正交实验对其提取工艺参数进行优化,并对多糖的抗氧化活性进行研究。结果表明,在提取次数为2次、乙醇用量为4倍体积时,最佳提取工艺参数为超声功率700 W、超声提取时间50 min、液固比为20∶1(m L·g^(-1)),此时双孢菇菇柄多糖得率可达5.35 g·100 g^(-1)。与抗坏血酸相比,双孢菇菇柄多糖具有较强的DPPH·清除能力,对·OH的清除能力和还原能力较弱。

In order to expend the utilization of Agarieus bisporus waste stipe, using A.bisporus waste stipe as raw materials, the effects of ultrasonic power, ultrasonic extraction time, liquid-solid ratio, ultrasonic extraction times and ethanol content on the yield of polysaccharldes from A. bisporus stipe were investigated by single factor experiment. Technology parameters were optimized by orthogonal test.And the antioxidant activities of polysaccharides in vitro were also assayed.Resuhs showed that the optimum extraction conditions were as follows：ultrasonic power was 700 W,ultrasonic extraction time was 50 min,liquid-solid ratio 20：1 （ mL·g^-1） ,2 times of ultrasonic extraction and volume of ethanol to extract for precipitation was 4 times.The yield of polysaccharides could be 5.35 g·100 g^-1.As compared to ascorbic acid,the polysaccharides of A.bisporus stipe had a powerful scavenging ability on DPPH·, a lower scavenging ability on hydroxyl radicals and reducing power.