喹赛多及其两种代谢产物在鸡体内消除规律研究

The Elimination of Cyadox and Its Two Major Metabolites in Chickens

通过喹赛多(Cyadox,CYX)的不同给药方式,研究CYX及其两种重要代谢产物在鸡体内消除规律,了解其对食品安全的影响,并为今后的药理学和毒理学研究提供较为详细的数据基础。建立并优化了喹赛多、脱二氧喹赛多(BDCYX)和喹啉-2-羧酸(QCA)在鸡血浆、胆汁、可食性组织和粪便中的提取、纯化及HPLC检测方法,并通过按推荐剂量连续混饲给药7 d和一次性灌胃给药两种不同给药方式,研究喹赛多及其两种代谢产物在鸡体内消除规律。结果表明,连续混饲给药7天后在血浆、肝脏、肾脏、肌肉、脂肪中均未检测到CYX,停药后6 h和24 h的肝脏样品中检测到BDCYX。除了胆汁以外,所检测的其它上述5种组织中均检测到QCA,肝脏和肾脏中持续检测到72 h。一次性灌服CYX后的鸡排泄物中CYX原形和BDCYX在给药后2 d便无法检出,QCA可检测到第3天。研究结果为喹赛多药理学和毒理学研究提供了可直接借鉴的参考数据。

The elimination characteristic of Cyadox （CYX） and its two major metabolites, 1,4-bisdesoxycyadox （BDCYX） and Quinoxalie- 2- carboxylie acid （QCA） were investigated in chickens for its study on pharmacology, toxicology and food safety concerns. In this study, a simple HPLC method was developed and validated for determination of CYX, BDCYX and QCA, following a group of 6 chickens （ 3 male and 3 female ） were administered a single oral dose of CYX （ 10 mg/kg b.w. ） , and feces were collected every 24 h after dose for the excretion studies. For the tissue elimination studies, 20 chickens were divided into five groups. Each groups consisted of 4 chickens, were fed with the diets containing CYX （ 100 mg/kg） for 7 consecutive days. One group of two male and two female chickens were randomly selected to be slaughtered at each time point （6 h, 1 d, 3 d, 7 d, 14 d） after withdrawal period. The results showed that CYX was non-detectable in plasma, liver, muscle, kidney and fat, while BDCYX was detectable at 6 h and 24 h in liver. QCA was detected in all selected four tissues and plasma, and detected to 72 h in liver and kidney. The CYX and BDCYX were detected to 2 d, and QCA was detected to 72 h in feces of chickens after a single oral gavage of CYX. These results provide comprehensive information for the food safety evaluation of CYX and will improve the understanding of the pharmacology and toxicology of CYX in animals.