摘要
建立了HPLC法测定注射用三氮脒中三氮脒含量的方法。采用C18色谱柱,以0.05 mol/L磷酸溶液-乙腈(95∶5,V/V)为流动相,流速为1.0 m L/min,检测波长为370 nm,柱温为30℃。结果三氮脒在1~100μg/m L浓度范围内线性关系良好,相关系数r=0.9998。建立的方法操作简便、准确、可靠,可用于三氮脒的质量控制。
A HPLC method was established for determination of diminazene aceturatein diminazene aceturate for injection. Analysis was performed on ODS chromatography column. The mobile phase was consisted of a mixture of 0.05 mol/L phosphate solution and acetonitrile （95 ： 5, V/V） . The deteced wavelength was 370 nm, the flow rate was 1.0 mL/min, and the column temperature was 30 ℃. A with the range of 1- 100μg/mL for diminazene aceturate（ r= good linearity of the calibration curves was obtained 0.9998）. The method is simple, accurate, reliable and applicable to the determination of diminazene aceturate in diminazene aceturate for injection.
出处
《中国兽药杂志》
2018年第1期46-49,共4页
Chinese Journal of Veterinary Drug
基金
浙江省"三农六方"科技协作项目(CTZB-F160728AWZ-SNY1-25)
