蓝莓种质资源遗传多样性的EST-SSR分析

Genetic diversity among blueberry cultivars:An EST-SSR analysis

文章采用EST-SSR分子标记对6个蓝莓品种进行遗传多样性分析。结果表明:利用13对多态性引物共扩增出65条条带,其中多态性条带53条,多态性比率为81.5%,多态性信息量(PIC)范围为0.513~0.887,平均每对引物的PIC值为0.698,遗传分化系数F_(st)平均值为0.137,基因流N_m的平均值为1.567,说明6种供试材料种群之间交流较大,遗传多样性较低,遗传基础相对狭窄,物种之间亲缘关系较近。

The genetic diversity among 6 different blueberry cultivars was analyzed by EST-SSR molecular markers.Sixty-five alleles including 53 polymorphic alleles were amplified with 13 pairs of primers.The polymorphism rate was 81.5%.The mean value of PIC（polymorphism information content） was 0.721（0.513-0.887）,the mean value of Fst（the coefficient of genetic differentiation） was 0.137,and Nm（gene flow） was 1.567.The results showed that there was a large gene flow among the 6 cultivars,the genetic diversity was low with relatively narrow genetic basis and the genetic relationship was close.