微RNA对脊髓损伤小鼠恢复的影响

Effect of microRNAs on recovery of spinal cord injury in mice

目的研究微RNA(miRNA)对脊髓损伤模型小鼠恢复的影响。方法查询、预测靶基因和miRNA并在293T细胞中验证;构建小鼠脊髓损伤模型并过表达miRNA,进行BBB评分,观察脊髓形态,检测标记基因及靶基因的表达。结果 miR-9、miR-124和miR-125在体外都能引起3个及以上靶基因的下调;miR-9和miR-124组小鼠脊髓钳夹伤口恢复较好;miR-9组小鼠运动功能恢复比对照组好,miR-124组比对照组差;miR-9组小鼠GFAP下调,MAP2和Neu N上调,靶基因GLIS3和CYBRD1下调。结论脊髓损伤小鼠模型中,miR-9可能通过靶向下调GLIS3和CYBRD1促进脊髓损伤的修复。

Objective To investigate the effect of microRNAs on the recovery of spinal cord injury in mice. Methods Target genes and miRNAs were searched and predicted, the results were confirmed in 293T cells. Spinal cord injury model was constructed in mice. The lentivirus-mediated miRNAs were injected via tail vein in mice with spinal cord injury. BBB behavioral experiment was performed and the morphology of spinal cord was observed. The expression of markers and target genes were detected. Results The miR-9, miR-124 and miR-125 induced the down-regulation of three or more target genes in vitro. miR-9 and miR-124 promoted the recovery of spinal cord injury caused by clamping; miR-9 group was better on movement function recovery, however, the effect of miR-124 was worse than that of control group. In miR-9 group, GFAP was down-regulated, MAP2 and NeuN were up-regulated, and the target genes GLIS3 and CYBRD1 were also down-regulated. Conclusion MiR-9 may promote the repair of spinal cord injury by down-regulating the expression of target genes GLIS3 and CYBRD1 in mice with spinal cord injury.