摘要
通过改进DNA提取方法,利用多层点样的电泳技术和样本量确定等方面,探讨SSR分子标记检测杂交稻种纯度技术的改良,以期能提高室内纯度检测的可靠性及效率。结果表明:利用TPS法提取糙米DNA和3层点样的电泳技术是可行的;192~288的样本量较适合室内种子纯度检测。
This paper discussed improvement of purity detection of hybrid rice seeds by SSR molecular markers from multiple aspects, such as optimization of the DNA extraction method,application of the multiple spotting electrophoresis technique and determination of the proper sample size, which may make the indoor purity detection more reliable and higher efficient.The results showed that it is feasi- ble to extract DNA of brown rice by TPS method and analyze DNA with third spotting in eleetropho- resis.Also,the sample size of 192--288 is suitable for detection of indoor seed purity .
出处
《种子》
北大核心
2018年第1期1-3,7,共4页
Seed
基金
广东农业种质资源共享利用服务平台构建与运行机制研究(2013B060400038)
广东省农作物种质资源鉴评利用公共服务平台建设(2016B030303007)
水稻籽粒萌发过程中糊粉层细胞器超微结构及其变化规律的研究(201429)
关键词
SSR分子标记
种子纯度
样本量
技术改良
SSR Molecular Marker
purity of seed
sample size
technique improvement
