颜色量化分析在大黄炭品质评价中的应用

Application of Color Quantitative Analysis in Quality Evaluation of Rhei Radix et Rhizoma Charcoal

目的：建立大黄炭颜色量化的评价方法,使主观抽象的感官评价标准化和科学化。方法：对生大黄及同批自制不同炮制程度的大黄炭样品着色、未着色外表面及着色、未着色粉末颜色R（红）,G（绿）,B（蓝）值（简称RGB值）进行测定,利用SPSS 19.0软件进行相关性分析。结果：每个样品各颜色RGB值间都呈现规律R值〉G值〉B值,总RGB值与相应的R值,G值,B值的趋势保持一致。通过相关性分析,着色前后外表面颜色间呈高度显著相关性,r=0.899,P〈0.01;且无论着色与否,外表面颜色与未着色粉末颜色间均呈显著相关性,相关系数分别为0.708和0.639,P〈0.05。结论：未着色粉末颜色总RGB值可客观反映大黄炭的颜色信息,所建立的大黄炭颜色量化分析方法让抽象的视觉颜色转换成了量化具象的RGB值。这种视觉颜色的成功创建让感官评价的量化和标准化成为可能,将有利于中药品质评价准确性的提高,也为其他中药感官评价的量化和标准化提供了一定的实验基础和参考。

Objective： To establish a quantitative evaluation method for the color of Rhei Radix et Rhizoma charcoal in order to standardize the subjective and abstract sensory evaluation. Method： The red,green and blue values（RGB values） about the outer surface and powder color of Rhei Radix et Rhizoma and Rhei Radix et Rhizoma charcoal with different processing degree were determined, colors included already colored and uncolored. Correlation analysis was conducted by SPSS 19. 0 statistical software. Result： Each color RGB values of each sample had the rule of R value G value B value,and the sum of RGB values was consistent with the corresponding values of R value, G value and B value. Through correlation analysis, there was a highly significant correlation between the color of the surface before and after colouring; and whether it＇s colored or not,there was a significant correlation between the outer surface color and the uncolored powder color,the correlation coefficients were 0. 708 and 0. 639. Conclusion： The sum of uncolored powder RGB values can objectively reflect the color information of Rhei Radix et Rhizoma charcoal. The method of quantitative analysis of the color of Rhei Radix et Rhizoma charcoal has been established to transform the abstract visual color into the quantified and figurative RGB values. The successful creation of this visual color makes the quantification and standardization of sensory evaluation possible,which will facilitate the improvement of the accuracy of traditional Chinese medicine（TCM） quality evaluation. At the same time,it also provides a certain experimental basis and reference for the quantitative and standardization of other TCM sensory evaluation.