臭氧与硫熏处理后当归品质变化情况的比较

Comparison of Quality Changes of Angelicae Sinensis Radix Treated by Ozone Gas and Sulfur Fumigation

目的：比较臭氧处理及硫熏处理后当归品质的变化情况,为臭氧处理作为硫熏处理替代技术的可行性分析提供参考。方法：采用间歇臭氧处理及硫磺熏蒸的方法分别处理当归药材,通过RP-HPLC测定当归中阿魏酸含量,流动相乙腈-0.085%磷酸水溶液（17∶83）,检测波长316 nm;采用水蒸气蒸馏法提取当归挥发油,通过GC-MS检测当归挥发油中化学成分;以微生物含量为指标,评价不同处理方式对当归储藏周期的影响。结果：臭氧处理及硫熏处理都会影响当归中阿魏酸的含量,较未处理当归样品中的质量分数分别降低了23.72%和34.43%;臭氧处理及硫熏处理当归后会导致挥发油颜色发生变化,且挥发油体积较未处理的样品分别降低了53.85%和76.92%,挥发油化学成分数量和种类均发生了较大的变化,主成分蒿本内酯分别降低了10.65%和9.32%;经储藏周期试验发现,臭氧处理后当归中的微生物含量较硫熏处理后的样品少。结论：当归经臭氧处理后化学成分变化程度较硫熏处理小,有望成为当归硫熏处理的替代技术。

Objective： To provide theoretical basis for the feasibility of ozone treatment technology as a select alternative technology to sulfur fumigation by comparing the quality of Angelicae Sinensis Radix after ozone treatment and sulfur fumigation treatment. Method： Angelicae Sinensis Radix was treated with intermittent ozone gas and sulfur fumigation process, respectively. The content of ferulic acid in Angelicae Sinensis Radix was determined by RP-HPLC. The volatile oil from Angelicae Sinensis Radix was extracted by steam distillation and its chemical constituents were determined by GC-MS. The microbial content was taken as the index,the effect of different treatments on the storage period of Angelicae Sinensis Radix was evaluated. Result： Compared with the untreated Angelicae Sinensis Radix,the content of ferulic acid decreased 23. 72% and 34. 43% after ozone and sulfur fumigation treatment, respectively. After ozone and sulfur fumigation treatment, the color, content and compositions of volatile oil from Angelicae Sinensis Radix were changed,the amount of volatile oil decreased by53. 85% and 76. 92%,the content of ligustilide decreased by 10. 65% and 9. 32%,respectively. The microbial content in Angelicae Sinensis Radix after ozone treatment was less than that of sulfur fumigation treatment.Conclusion： The changes in chemical composition of Angelicae Sinensis Radix treated with ozone are less than that of sulfur fumigation treatment. It is expected to be an alternative to sulfur fumigation treatment of Angelicae Sinensis Radix.