HPLC-DAD波长转换法同时测定不同产地滇桂艾纳香中4种成分的含量

Simultaneous Determination of Four Components in Blumea riparia by HPLC with Wavelength Switching

目的：建立滇桂艾纳香药材中原儿茶酸、绿原酸、咖啡酸和芦丁4种成分的含量测定方法,同步测定4种成分含量,为滇桂艾纳香药材的质量控制提供参考。方法：采用HPLC转换波长法,Inertsil ODS-3色谱柱（4.6 mm×250 mm,5μm）,以乙腈（A）-0.1%磷酸水溶液（B）为流动相进行梯度洗脱（0~25 min,10%~18%A;25~45 min,18%~40%A）,检测波长256 nm（0~15 min,原儿茶酸）,327 nm（15~30 min,绿原酸、咖啡酸）,360 nm（30~60 min,芦丁）,柱温20℃,流速1.0 m L·min^-1。结果：滇桂艾纳香中原儿茶酸、绿原酸、咖啡酸、芦丁进样量分别在0.007~0.13μg（r=0.999 98）,0.219~4.38μg（r=0.999 94）,0.014~0.28μg（r=0.999 97）,0.049~0.98μg（r=0.999 97）与峰面积呈良好的线性关系,平均加样回收率分别为98.84%（RSD 2.2%）,101.16%（RSD 0.9%）,98.00%（RSD 2.8%）,101.73%（RSD 1.4%）。含量测定结果显示,百色平果县所产药材中原儿茶酸、绿原酸和芦丁含量均较高,百色靖西县和四川药材咖啡酸含量较高。结论：所建立的方法稳定可靠,灵敏度高,重复性好,可同时测定滇桂艾纳香药材中原儿茶酸、绿原酸、咖啡酸和芦丁4种成分的含量,可为滇桂艾纳香药材质量评价提供参考。

Objective： To determinate the contents of protocatechuic acid,chlorogenic acid,caffeic acid and rutinin in Blumea riparia by HPLC,and provide references for its quality control. Method： The HPLC analysis was carried out by Inertsil ODS-3 column（4. 6 mm × 250 mm,5 μm） with acetonitrile（A）-0. 1% phosphoric acid solution（B） as the mobile phase for gradient elution. The detection wavelength was set at 256 nm（0-15 min,protocatechuic acid）,327 nm（15-30 min,chlorogenic acid and caffeic acid）,and 360 nm（30-60 min,rutin）.In addition,the flow rate was 1. 0 m L·min^-1 and column temperature was 20 ℃. Result： Protocatechuic acid,chlorogenic acid,caffeic acid and rutin showed good linearity in the range of 0. 007-0. 13 μg（r = 0. 999 98）,0. 219-4. 38 μg（r = 0. 999 94）,0. 014-0. 28 μg（r = 0. 999 97） and 0. 049-0. 98 μg（r = 0. 999 97）respectively,and the recovery of those 4 components were 98. 84%（RSD 2. 2%）,101. 16%（RSD 0. 9%）,98. 00%（RSD 2. 8%） and 101. 73%（RSD 1. 4%） respectively. The content determination results showed that the contents of protocatechuic acid,chlorogenic acid and rutinin were higher in Pingguo samples; and the content of caffeic acid was higher in Jingxi and Sichuan samples. Conclusion： The established method can be used to determine the contents of protocatechuic acid,chlorogenic acid,caffeic acid and rutin. Such stable,reliable and repeatable analytical model can provide a reference for the quality evaluation and its control on Blumea riparia.