甘肃一、二年生红芪与黄芪黄酮类成分指纹图谱分析

Flavonoids Fingerprint of One or Two Years Old Hedysari Radix and Astragali Radix

目的：建立甘肃不同产地一、二年生红芪和黄芪中黄酮类成分的指纹图谱,为科学评价甘肃红芪和黄芪的内在质量提供参考。并阐明红芪和黄芪能否替代使用的依据。方法：应用HPLC,测定甘肃一年生、二年生各28批红芪和28批黄芪黄酮类成分指纹图谱。色谱条件：Agilent ZROBAX-C18色谱柱,流动相乙腈-0.1%甲酸水溶液,梯度洗脱,流速1.0 m L·min^-1,检测波长254 nm,柱温35℃。结果：甘肃各产地一、二年生28批红芪有19个共有峰,28批黄芪有14个共有峰,一年生28批红芪和28批黄芪有9个共有峰,二年生28批红芪和28批黄芪有8个共有峰。甘肃各产地一年生红芪平均相似度为0.976,二年生红芪平均相似度为0.888;甘肃各产地一年生黄芪平均相似度为0.986,二年生黄芪平均相似度为0.911,一年生红芪和黄芪相似度为0.534,二年生红芪和黄芪相似度位0.490。结论：该方法重复性好,建立的指纹图谱为甘肃红芪和黄芪的质量控制及评价提供依据。随着药材生长年限的增加,甘肃红芪相似度降低,黄芪相似度降低,红芪和黄芪相似度降低。从红芪和黄芪黄酮类成分指纹图谱相似度来看,二者一年生和二年生的相似度均很低,差异较大,不适合替代使用。

Objective： To establish the flavonoids fingerprints of one or two years old Hedysari Radix and Astragali Radix from different areas in Gansu province,provide reference for scientific evaluation of the intrinsic quality of Gansu Hedysari Radix and Astragali Radix,and clarify the basis on whether Hedysari Radix and Astragali Radix can be alternatively used. Method： HPLC was used to determine the flavonoids fingerprints of 28 batches of one or two years Hedysari Radix and 28 batches of one or two years Astragali Radix in Gansu. Chromatographic conditions： Agilent ZROBAX-C18,mobile phase acetonitrile-0. 1% formic acid aqueous solution,gradient elution,flowrate 1. 0 m L·min^-1,detection wavelength 254 nm,and column temperature 35 ℃. Result： The flavonoids fingerprint showed 19 common peaks in one or two years old Hedysari Radix,and 14 common peaks in one or two years Astragali Radix,9 common peaks in one year old Astragali Radix and Hedysari Radix,and 8 common peaks in two years old Astragali Radix and Hedysari Radix. Among them,the average similarity was 0. 976 in one year Hedysari Radix from different areas,0. 888 in two years Hedysari Radix,0. 986 in one year Astragali Radix from different areas,and 0. 911 in two years Astragali Radix; the similarity was 0. 534 between one year Hedysari Radix and Astragali Radix,and 0. 490 between two years Hedysari Radix and Astragali Radix. Conclusion： The method is reproducible and the established fingerprints provide the basis for the quality control and evaluation of Gansu Hedysari Radix and Astragali Radix. The similarity of Hedysari Radix and Astragali Radix in Gansu was very low,and the similarity decreased with the increase of age,so these two samples are not suitable for alternative use.