YAP、LPA及TAZ在头颈鳞癌中的表达及其与临床病理特征的关系

Expression of YAP,LPA and TAZ in head and neck squamous cell carcinoma and its correlation with clinicopathological data

目的探讨溶血磷脂酸(LPA)、Yes相关蛋白(YAP)及含PDZ结合区的共转录激活因子(TAZ)在头颈鳞癌中的表达及其与临床病理特征的关系。方法选择性收集雅安市人民医院病理科2010年1月至2016年12月期间,手术切除的病理组织石蜡标本共计136例,其中包括头颈鳞癌癌旁正常组织37例、头颈良性瘤样组织35例、头颈鳞癌64例,所有标本术前均无放化疗史,采用免疫组化及实时定量PCR检测LPA、YAP及TAZ在各组织中的表达及临床相关参数的关系。结果 LPA m RNA在头颈鳞癌癌旁正常组织、良性瘤样组织和鳞癌组织中的表达相近(P>0.05);YAP m RNA在鳞癌组织中的表达要高于癌旁组织、瘤样组织中的表达(P<0.05);TAZ m RNA在头颈3种组织中呈低表达,但鳞癌组织的表达明显高于良性瘤样组织及癌旁正常组织(P<0.05);YAP及TAZ蛋白在头颈鳞癌组织中的表达显著高于在癌旁正常组织和良性瘤样组织中的表达(P<0.05);鳞癌患者YAP的表达在肿瘤>3.0 cm、淋巴结阳性显著高于对照组(P<0.05);TAZ的表达在肿瘤中低分化、肿瘤>3.0 cm、淋巴结阳性及Ⅲ~Ⅳ期高于对照组(P<0.05)。结论 YAP及TAZ在头颈鳞癌中的表达与肿瘤分期、分化程度、淋巴结转移密切相关,而LPA的表达无显著差异及相关性。

Objective To investigate the expression of YAP, LPA and TAZ in head and neck squamous cell carcinoma and its clinical significance. Methods Selective collection of Ya＇an People＇s Hospital pathology department from January 2010 to December 2016, the surgical resection of the pathological tissue paraffin specimens amounted to 136 cases, including the normal tissue of head and neck squamous cell carcinoma 37 cases, head and neck benign tumor-like tissue 35 cases, head and neck squamous cell carcinoma 64 cases, all specimens without chemotherapy history, The relationship between the expression of LPA, YAP and TAZ in various tissues by immu- nohistoehemistry and real-time quantitative PCR assay and its clinical correlative parameters. Results The expres- sion of LPA mRNA in normal tissues, benign neoplasia tissues and squamous carcinoma tissues of the head and neck squamous cell carcinoma was similar （P 〉 0.05 ）. The expression of YAP mRNA in squamous cell carcinoma tissues was higher than that in the adjacent tissues and tumor tissues （P 〈 0.05）. TAZ mRNA was low expression in the three tissues of the head and neck, but the expression of squamous cell carcinoma was significantly higher than that of benign tumor tissues and adjacent normal tissues （P 〈 0.05）. The expression of YAP and TAZ protein was significantly higher than that in the tissues of normal tissues and benign tumor tissues （P 〈 0.05）. The expres- sion of YAP in squamous cell carcinoma was significantly higher than the control group （P 〈 0.05）. The expression of TAZ was low differentiation in tumor, tumor 〉3.0 cm, lymph node positive and III-IV period were higher than the control group （P 〈 0.05）. Conclusions The expression of YAP and TAZ in head and neck squamous cell car- cinoma was closely related to tumor staging, differentiation degree and lymph node metastasis, while the expression of LPA showed no significant difference and correlation.