某氡温泉周围居民外周血淋巴细胞周期及其调控蛋白的表达

Cell cycle and its regulatory proteins in the peripheral blood lymphocytes of the residents living in a radon hot spring area

目的 观察某氡温泉周边居民外周血淋巴细胞周期各时相变化以及周期调控蛋白CDK1、CDK2、CDK4、CDK6、CyclinD1、CyclinE1、WEE1和CDC25A的表达。方法 简单随机抽样法抽取温塘镇氡温泉周边居民46人，抽取对照地区居民39人。流式细胞术分别检测细胞周期各时相的变化和周期调控蛋白的表达水平。比较两组细胞周期各时相的差异以及周期调控蛋白表达水平的差异，多重线性回归分析周期调控蛋白表达与氡暴露等因素的关系。结果 G0/G1期和S期细胞所占比例在两组比较中有差异（t=2.250、-2.382，P〈0.05）；CDK1、CDK6和CyclinE1在氡温泉组中的表达水平显著下调（t=4.770、11.419、5.238，P〈0.05），并与氡暴露因素存在相关关系且呈负相关（t=-5.097、-11.128、-5.117，P〈0.05）。CDK2、CDK4、CyclinD1、WEE1和CDC25A在氡温泉组表达均上调，但差异无统计学意义（P〉0.05）。结论 氡周边居民外周血淋巴细胞S期比例增加，周期调控蛋白CDK1、CDK6和CyclinE1表达下调，可能与长期的氡暴露有关。

Objective To observe potential effect of radon hot springs on the changes of cell cycle and its regulatory proteins of CDK1, CDK2, CDK4, CDK6,CyclinD1, CyclinE1, WEE1, CDC25A in the peripheral blood lymphocytes of residents. Methods A random sampling method was used to persons 46 persons from the residents around radon hot spring in Wentang town, and 39 persons were selected from the control area without radon exposure. Flow cytometry was used to detect the changes of cell cycle and the expressions of cell cycle-related regulatory proteins. Multiple linear regression method was used to analyze the relationship between the expressions of cell cycle regulatory proteins and radon exposure. Results The percentages of cells at G0/G1 phase and S phase in lymphocytes were different in the two groups （t=2.250, -2.382, P〈0.05）. The expression levels of CDK1, CDK6 and CyclinE1 in the peripheral blood lymphocytes of radon hot spring group were significantly decreased （t=4.770,11.419,5.238,P〈0.05） and negatively correlated with radon exposure （t=-5.097, -11.128,-5.117, P〈0.05）.The expression levels of CDK2, CDK4, CyclinD1, WEE1 and CDC25A in the peripheral blood lymphocytes of radon hot spring group were increased but not significantly（P〉0.05）. Conclusions The incidences of a higher ratio of S-phase cells and lower expression levels of CDK1, CDK6 and CyclinE1 in the peripheral blood lymphocytes of residents in Wentang town may be related to long-term radon exposure