培哚普利改善心室重构作用与Gal-3的相关性

The association between Gal-3 and the effect of perindopril on ventricular remodelin

目的探讨培哚普利改善缺血性心力衰竭家兔心室重构作用与半乳糖凝集素-3(Gal-3)的相关性。方法采用结扎冠状动脉前降支的方法制作缺血性心力衰竭家兔模型。将30只家兔分为假手术组、心力衰竭组和培哚普利组。给药4周后心脏超声测定心功能;分别用实时荧光定量PCR和Western blot方法检测心肌组织的Gal-3、Ⅰ型胶原、Ⅲ型胶原的mRNA表达和蛋白水平;ELISA检测家兔血清Gal-3水平。结果与假手术组相比,心力衰竭组梗死区心肌组织中Gal-3、Ⅰ型胶原、Ⅲ型胶原mRNA的表达与蛋白水平增加(P<0.05)、血清Gal-3水平上升(P<0.05);与心力衰竭组相比,培哚普利组Ⅰ型胶原、Ⅲ型胶原mRNA的表达和蛋白水平降低(P<0.05),与此同时Gal-3mRNA的表达和蛋白水平降低(P<0.05),血清Gal-3水平降低(P<0.05);且Gal-3水平与心功能呈负相关(r=-0.925,P<0.05)。结论培哚普利抑制心肌纤维化,减缓心室重构过程,改善心功能的作用与Gal-3水平降低有关。

Objective To investigate the association between Gal-3 and the effect of perindopril on ventricular remodeling in ischemic heart failure rabbit. Methods A rabbit model of ischemic heart failure was made by ligation of the anterior descending branch of the coronary artery. Thirty rabbits were divided into sham operation group,heart failure group and perindopril group. Determination of cardiac function by echocardiography after 4 weeks of treatment respectively;mRNA expression and protein content of Gal-3 were detected by Real-time PCR or Western-blob. Serum Gal-3 level Was determinated by ELISA. Results Compared with sham operation group,mRNA expression and protein content of Gal-3,type I collagen and type III collagen increased and the serum level of Gal-3 increased in heart failure group（P〈0. 05） ;compared with heart failure group,mRNA expression and protein content of Gal-3,type I collagen and type III collagen decreased and the serum level of Gal-3 was reduced in perindopril group（P〈0.05）. Gal-3 was negatively correlated with heart function（r=-0. 925 ,P〈0.05）. Conclusion Effect of perindopril inhibiting myocardial fibrosis,slowing the ventricular remodeling and improving heart function associated with level of Gal-3.