贵阳地区2015年手足口病柯萨奇病毒A6、A10型的检测分析

Analysis of Coxsackie virus A6 and A10 detection in hand foot and mouth disease in Guiyang area during 2015

目的了解贵阳地区2015年引起手足口病(HFMD)的柯萨奇病毒(CV)A6、CVA10型肠道病毒(EV)的病原构成情况,为本地区HFMD防治提供依据。方法根据GenBank上的CVA6和CVA10序列,分别设计特异性引物,建立荧光定量RT-PCR方法体系,用基因测序法进行验证,然后利用该方法对607例HFMD临床病例标本进行检测。结果共检测HFMD疑似病例607例,总阳性率为59.47%(361/607),其中EV71占7.25%(44/607),CVA16占11.37%(69/607),EV71和CVA16双阳性占0.16%(1/607),其他EV占40.69%(247/607)。用建立的实时荧光RT-PCR法检测出CVA6、CVA10和CVA6+CVA10型阳性样本分别为11、71和1例[总阳性率13.67%(83/607)],且与基因测序法结果完全符合。结论 CVA6、CVA10是贵阳地区2015年HFMD新发的主要病原,应加强对其型别的监测。

Objective To understand the enterovirus（EV） pathogenic composition of CoXsackie virus（CV）A6 and A10 causing hand foot and mouth disease（HFMD） in Guiyang area during 2015 to provide a basis for the prevention and treatment of HFMD in local area. Methods The specific primers were respectively designed according to CVA6 and CVA10 sequence published in GenBank. The fltiorescence quantitative RT-PCR method system was established. The gene sequencing method was used for conducting verification. Then this method was used to detect the clinical samples from 607 cases of HFMD. Results A total of 607 samples of suspected HFMD were detected,the overall positive rate was 59.47 % （361/607）,in which EV71 accounted for 7.25 % （44/607） ,CVA16 for 11.37%（69/607）,EV71-kCVA16 double positive accounted for 0.16%（1/607） and other EV for 40.69% （247/607）. The positive samples of CVA6 ,CVA10 and CVA6＋CVA10 detected by the established real time fluorescence RT-PCR were 11 cases, 71 cases and 1 case. Conclusion CVA6 and CVA10 are the main pathogens causing new onset HFMD in Guiyang area and the CVA10 monitoring should be strengthened.