尿液荧光光谱对胃癌诊断初步研究

Preliminary Study on Diagnosis of Gastric Cancer by Urine Fluorescence Spectroscopy

目的通过对胃癌和健康者的尿液进行荧光检测,建立胃癌的快速筛查方法,为胃癌患者的早期诊断及其生存率提高提供实验依据。方法采用激发波长395 nm分别对健康者、放化疗前后胃癌患者的尿液光谱进行检测,并对检测后的数据进行分析。结果胃癌患者尿液荧光光谱在发射波长615 nm附近出现特征峰,且615 nm处荧光强度与其相邻波谷599 nm荧光强度呈负相关;依据615 nm和599 nm处的相对荧光强度,定义指标T=(I615-I599)/I599,,建立胃癌的诊断标准:T>0.1,I599≤25,不满足条件的为阴性,利用此诊断指标对26名胃癌患者、23名健康者进行双盲实验,发现阳性符合率为69.23%,假阳性率为17.39%。通过与标准物对比,图谱产生特征峰的物质可能是粪卟啉。结论胃癌患者粪卟啉代谢异常,尿液中粪卟啉含量升高,可作为胃癌早期诊断的指标。

Objective To establish a rapid screening method for gastric cancer by fluorescence detection and provide an experimental basis for early diagnosis of gastric cancer and improvement of survival rate. Methods Using excitation wavelength 395nm to detected urine spectra of healthy people and gastric cancer patients and analysing the date. Results Fluorescence spectra of urine in patients with gastric cancer had a small peak in the vicinity of the emission wavelength of 615nm,furthermore, it had a negatively correlated that with the fluorescence intensity at 599nm.At the same time, according to the relative fluorescence intensity of 615nm and 599nm, build the definition index of T= （I615-I599）/I599and establish diagnostic criteria of gastric cancer： T〉0.1, I599≤25, using this diagnostic index of double-blind experiments on 26 patients with gastric cancer and 23 healthy people, we found that the positive rate was 69.23 %, the false positive rate was 17.39 %. In addition, it is possible to investigate the substances that produce characteristic peaks, which may be fecal porphyrins by comparing them with standard substances. Conclusion The abnormal metabolism of porphyrin in urine of gastric cancer patients, as the tumor cell appearing, more and more porphyrins are produced in urine, which can be used as an indicator for early diagnosis of gastric cancer.