高效液相色谱法同时测定化妆品中12种防腐剂

Simultaneous Determination of 12 Preservatives in Cosmetics by HPLC

采用高效液相色谱法同时检测化妆品中12种防腐剂的含量。C18色谱柱(250 mm×4.6 mm×5μm)为分析柱,甲醇+乙腈+0.02 mol·L-1磷酸二氢钠(pH=3.5)为流动相,梯度洗脱。2-溴-2-硝基丙烷-1,3-二醇和苯甲醇检测波长为210 nm;甲基氯异噻唑啉酮和甲基异噻唑啉酮检测波长为280 nm;其它组分检测波长为254 nm。结果表明各组分在浓度为4.242~299.0 mg/L范围内与目标峰面积均呈较好的线性关系,R均大于0.9999。加标回收率在92.6%~108%之间,各组分的相对标准偏差(n=7)在1.2%~3.1%之间。方法相对《化妆品卫生规范》相比,更简单、易操作、实用性更强,适用于化妆品中多种防腐剂的同时测定。

HPCL was developed for the determination of 12 preservatives in cosmetics. The sample was separated with a mixture of methanol, acetonitrile and 0.02 mol·L^-1 NaH2PO4 as mobile phase on a C18 column （250min×4.6mm×5μm） with gradient elution. The detection wavelength was 210 nm for 2-bromo-2-nitropropane-1,3-diol and benzyl alcohol, 280 nm for metylchloro isothiazolinone and methylisothiazolinone, and 254 nm for others. Good linear relationship between values of peak area and concentration of preservatives was obtained in the range from 4.242 to 299.0 mg/L, and the correlation coefficients were above 0.9999. The recovery rate ranged 92.6 % from 108 %, and relative standard deviations （RSD） （n=7） were between 1.2 % and 3.1%. The method is simpler, easier to operate, more practical compared to Hygienic Standard for Cosmetics and can be applied to the determination of 12 preservatives in cosmetics simultaneously.