气相色谱法测定红色正金软膏中薄荷脑的含量

Determination of Menthol Content in Compound Red Gold Ointment by Gas Chromatography

目的建立红色正金软膏中薄荷脑含量测定的方法。方法采用气相色谱法测定红色正金软膏中薄荷脑的含量,色谱条件:HP-INNOWAX(0.25 mm×30 m,0.25μm)色谱柱;进样口温度250.0℃;初始柱温110.0℃,恒温5.0 min,以20.0℃/min升温至220.0℃,恒温4.0 min;载气为高纯氮气;FID检测器(280.0℃);分流比为10.0。结果薄荷脑在浓度0.005~0.030 g/m L范围内有良好的线性关系(R2=0.9999),重复性试验的RSD为0.309%,精密度试验的RSD为0.608%,稳定性试验的RSD为1.430%,加样回收率为99.16%~101.47%,RSD=0.897%。结论气相色谱法测定红色正金软膏中薄荷脑含量快速、简便、准确。

Objective To establish GC method for determining the menthol content of compound red gold ointment. Methods Determination of menthol content in compound red gold ointment by gas chromatography. Chromatographic conditions： HP-INNOWAX（0.25 mm×30 m,0.25μm） chromatographic column, inject temperature 165.0 ℃; Initial column temperature 110.0 ℃, kept 5.0 rain, warming to 220 ℃ with 20.0℃/min, kept 4.0 min; high purity nitrogen as carrier gas; F ID temperature 280 ℃; split ratio was 10. Results Menthol showed good linear relationships within the range of 0.005-0.030 g/mL （R2=0.9999）; RSD of repeatability test was 0.309 %; RSD of precision test was 0.608 %; RSD of stability test was 1.430 %; recovery rate was 99.16 %-101.47 % （RSD=0.897 %）. Conclusions The GC method is rapid, simple and accurate for determination of menthol content in compound red gold ointment.