牛磺酸对大鼠脑缺血—再灌注时缺血区脑组织小胶质细胞活性的影响

The efficacy of taurine on microglial activation in ischemic brain tissue of rats during cerebral ischemia reperfusion damage

目的观察牛磺酸是否能通过抑制脑缺血—再灌注时小胶质细胞激活,抑制炎性反应,发挥保护脑组织的作用。方法 2016年3月—2017年5月在首都医科大学北京市神经外科研究所进行实验。SD大鼠54只,6只用于制备栓子,余48只大鼠随机分4组各12只,缺血2 h再灌注4 h对照Ⅰ组,缺血2 h再灌注4 h给药Ⅰ组,缺血2 h再灌注22 h对照Ⅱ组,缺血2 h再灌注22 h给药Ⅱ组。应用血栓栓塞法制作大鼠短暂性局灶性脑缺血—再灌注动物模型,应用免疫组织化学方法检测主要组织相容性复合体(MHC)抗原,观察牛磺酸对局灶性脑缺血2 h后再灌注4 h及22 h小胶质细胞活性的影响。结果与对照组Ⅰ比较,给药Ⅰ组缺血侧灰质区、胼胝体区及尾壳核区含MHCⅠ、MHCⅡ抗原阳性小胶质细胞数目明显降低(t_(MHCⅠ)=3.594、5.169、4.269,t_(MHCⅡ)=3.511、3.087、4.743,P<0.01或0.05);与对照Ⅱ组比较,给药Ⅱ组缺血侧灰质区、胼胝体区、尾状壳核区、视神经区含MHCⅠ、MHCⅡ抗原阳性小胶质细胞数亦明显降低(t_(MHCⅠ)=11.691、13.262、6.070、1.914,t_(MHCⅡ)=30.533、38.554、5.913、2.311,P<0.01或0.05);结论牛磺酸可通过抑制脑缺血—再灌注时小胶质细胞激活,发挥对脑组织的全脑保护作用。

Objective To investigate whether taurine could reduce microglial activation and inhibit inflammatory to protect brain tissue against cerebral ischemia reperfusion damage. Methods The experiment.was carried out in Capital Medical University Beijing Neurosurgery Research Institute From Mar. 2016 to May 2017.6 rats of 54 were used to prepare emboli, other 48 rats was randomly divided into 4 groups with 12 rats in each group： 2 h ischemia following 4 h reperfusion control group Ⅰ , 2 h ischemia following 4h reperfusion administration group 1 ,2 h ischemia following 22 h reperfusion control group Ⅱ , 2 h ischemia following 22h reperfusion administration group Ⅱ. Thromboembolic model of transient focal cerebral ischemia reperfusion was induced in the 48 rats. The efficacy of taurine on microglial activation at 2 hours ischemia following 4 and 22 hours of reperfusion was evaluated by major histocompatibility complex （MHC） antigen immunohistochemistry. Results Compared with control group Ⅰ , administration group Ⅰ could reduce numerical density of the MHC class Ⅰ immunopositive microglia in ischemic gray matter, corpus callosum and caudate putamen （ tMHCⅠ=3. 594,5. 169,4. 269, P 〈 0. 01 ; tMHCⅡ=3. 511, 3. 087,4. 743 ,P 〈0.05 ）. Compared with control group Ⅱ, administration group Ⅱ could reduce numerical density of the MHC class Ⅱ immunopositive microglia in ischemic gray matter, corpus callosum and caudate putamen（ tMnct = 11. 691, 13. 262,6. 070,1. 914 ,P 〈0.01 ;tMHCⅡ = 30. 533,38. 554,5. 913,2. 311 ,P 〈 0.05 ）. Taurine could reduce numerical density of the MHC class Ⅰand class Ⅱ immunopositive microglia in ischemic gray matter and white matter. Conclusion Taurine can inhibit inflammatory reactions from damage induced by cerebral ischemia reperfusion and exert its neuroprotective effect on brain tissue.