银杏叶提取物联合糖皮质激素对变应性鼻炎小鼠嗅觉功能及炎症因子的影响

Effects of ginkgo biloba extract combined with glucocorticoid on olfactory function and inflammatory cytokines in mice with allergic rhinitis

目的:研究银杏叶提取物联合糖皮质激素是否可以显著改善变应性鼻炎伴嗅觉障碍小鼠嗅黏膜的炎症反应,对变应性鼻炎所致嗅觉障碍起到治疗作用。方法:利用卵清蛋白对实验组小鼠进行造模,选取变应性鼻炎伴嗅觉障碍的30只小鼠分为3组:组1给予地塞米松和大剂量银杏叶提取物(n=10);组2给予地塞米松和小剂量银杏叶提取物(n=10);组3仅给予地塞米松(n=10)。连续给药两周。观察嗅黏膜形态结构的改变,比较小鼠血清中细胞因子IL-4、IFN-γ、caspase-1的检测结果。结果:治疗后,组1小鼠嗅觉显著强于组3小鼠(P<0.05)。各组caspase-1水平第2周较第1周均有显著提高。各组小鼠IFN-γ水平第2周较第1周均有显著降低。组1及组2小鼠IL-4水平第2周较第1周明显降低。各组IFN-γ/IL-4水平第2周较第1周均有显著降低。结论:与单纯应用糖皮质激素治疗相比,糖皮质激素联合银杏叶提取物具有更好的抗炎作用,可以在一定程度上提高变应性鼻炎所致嗅觉障碍的治疗效果。

Objective：To determine whether ginkgo biloba extract（GBE）combined with dexamethasone（DEX）plays a role in the treatment of allergic rhinitis-related olfactory dysfunction using an animal model.Method：Six week old BALB/C mice were sensitized and challenged with ovalbumin.30 sensitized mice were divided into three groups：Group 1 was given high-dose GBE and DEX（n=10）;Group 2 was given low dose GBE and DEX（n=10）;Group 3 was given DEX alone（n=10）.We assessed the histology of the olfactory mucosa and serum IL-4,IFN-γ,and caspase 1.Result：A significant higher fraction of mice in group 1 could find the food pellet within300 scompared to group 3（P0.05）.Caspase-1 levels improved during the second week compared with the first week in each group.IFN-γlevels were significantly lower during the second week compared with the first week（P0.05,all）.IL-4 levels also were significantly lower during the second week compared with the first week in all groups except those receiving DEX alone.IFN-γ/IL-4 levels in each group were significantly lower during the second week compared with the first week（P0.05,all）.Conclusion：In this animal model of allergic rhinitis-related olfactory dysfunction,the addition of ginkgo biloba extract to dexamethasone have a better anti-inflammatory effect,which can partly improve the therapeutic effect on olfactory dysfunction caused by allergic rhinitis.