临床实验室质谱检测原理

Clinical mass spectrometry:principles of mass spectrometry for clinical laboratories

质谱(MS)技术具有较高的特异性、灵敏度及可同时检测多个化合物的能力,对其的应用正成为临床实验室一个快速发展的领域。目前,已有多种MS分析仪应用于临床实验室,其检测性能正在快速提升。文章对MS技术作了基本介绍,包括临床实验室MS检测的相关仪器、电离技术及样品制备等,并就临床实验室开展MS检测所面临的挑战及应用前景进行探讨。

Mass spectrometry （MS） is a fast growing area in clinical laboratories due to its exceptional specificity,high sensitivity and ability to detect multiple compounds simultaneously. Several types of mass spectrometers are being used in clinical laboratories globally. As a result,tremendous improvements in assay performance are in process. This review is intended as a basic introduction to MS and describes the history of MS in clinical laboratories,instrumentation,ionization techniques and sample preparation. The review also discusses challenges to implementation in clinical laboratories and discussions on future instrumentation and future uses of MS.