Del-1对特异性过敏患儿嗜酸性粒细胞表面ICAM-1表达的影响

Influence of Del-1 on ICAM-1 expression of eosinophils in atopic allergy children

目的研究发育内皮基因1(Del-1)对特异性过敏患儿嗜酸性粒细胞上细胞间黏附分子1(ICAM-1)表达的影响,并初步探讨其意义。方法收集35例特异性过敏患儿外周血标本,利用磁珠分选(MACS)方法分选嗜酸性粒细胞,用Del-1、抗Del-1抗体或白细胞介素17(IL-17)处理,用逆转录聚合酶链反应(PCR)检测ICAM-1的表达,并进行统计学分析。结果与对照组比较,Del-1处理组ICAM-1表达水平下降(P<0.05),抗Del-1抗体处理组ICAM-1表达水平上升(P<0.05);Del-1与IL-17共同处理组ICAM-1表达水平较单独IL-17处理组下降(P<0.05);但在非嗜酸性粒细胞中,Del-1或抗Del-1抗体处理组ICAM-1表达水平较对照组[用磷酸盐缓冲液(PBS)处理]差异无统计学意义(P>0.05)。结论 Del-1可调节嗜酸性粒细胞表面的ICAM-1表达,提示Del-1可能通过抑制嗜酸性粒细胞的黏附作用抑制特异性过敏炎症。

Objective To evaluate the influence of developmental endothelial locus-1 （Del-1） on intercellular cell adhesion molecule-1 （ICAM-1）expression of eosinophils in atopic allergy children. Methods Peripheral blood samples were collected from atopic allergy children （35 cases）,and eosinophils were isolated by magnetic-activated cell sorting （MACS）. ICAM-1 expression was determined by reverse transcription polymerase chain reaction （PCR） after administrating Del-1,anti-Del-1 antibody or interleukin 17 （IL-17）. Results Compared with control group treated with phosphate-buffered saline （PBS）,ICAM-1 expression was down-regulated after administrating Del-1（P〈0.05）,and it was up-regulated after administrating anti-Del-1 antibody （P〈0.05）. ICAM-1 expression was down-regulated after administrating Del-1 and IL-17 compared with that after administrating IL-17 only （P〈0.05）. There was no statistical significance in non-eosinophils after administrating Del-1 or anti-Del-1 antibody （P〉0.05）. Conclusions Del-1 can regulate the expression of ICAM-1,indicating that Del-1 can negatively regulate the intercellular adhesion of eosinophils,thereby limiting atopic allergy of children.