摘要
目的研究发育内皮基因1(Del-1)对特异性过敏患儿嗜酸性粒细胞上细胞间黏附分子1(ICAM-1)表达的影响,并初步探讨其意义。方法收集35例特异性过敏患儿外周血标本,利用磁珠分选(MACS)方法分选嗜酸性粒细胞,用Del-1、抗Del-1抗体或白细胞介素17(IL-17)处理,用逆转录聚合酶链反应(PCR)检测ICAM-1的表达,并进行统计学分析。结果与对照组比较,Del-1处理组ICAM-1表达水平下降(P<0.05),抗Del-1抗体处理组ICAM-1表达水平上升(P<0.05);Del-1与IL-17共同处理组ICAM-1表达水平较单独IL-17处理组下降(P<0.05);但在非嗜酸性粒细胞中,Del-1或抗Del-1抗体处理组ICAM-1表达水平较对照组[用磷酸盐缓冲液(PBS)处理]差异无统计学意义(P>0.05)。结论 Del-1可调节嗜酸性粒细胞表面的ICAM-1表达,提示Del-1可能通过抑制嗜酸性粒细胞的黏附作用抑制特异性过敏炎症。
Objective To evaluate the influence of developmental endothelial locus-1 (Del-1) on intercellular cell adhesion molecule-1 (ICAM-1)expression of eosinophils in atopic allergy children. Methods Peripheral blood samples were collected from atopic allergy children (35 cases),and eosinophils were isolated by magnetic-activated cell sorting (MACS). ICAM-1 expression was determined by reverse transcription polymerase chain reaction (PCR) after administrating Del-1,anti-Del-1 antibody or interleukin 17 (IL-17). Results Compared with control group treated with phosphate-buffered saline (PBS),ICAM-1 expression was down-regulated after administrating Del-1(P〈0.05),and it was up-regulated after administrating anti-Del-1 antibody (P〈0.05). ICAM-1 expression was down-regulated after administrating Del-1 and IL-17 compared with that after administrating IL-17 only (P〈0.05). There was no statistical significance in non-eosinophils after administrating Del-1 or anti-Del-1 antibody (P〉0.05). Conclusions Del-1 can regulate the expression of ICAM-1,indicating that Del-1 can negatively regulate the intercellular adhesion of eosinophils,thereby limiting atopic allergy of children.
出处
《检验医学》
CAS
2018年第1期67-71,共5页
Laboratory Medicine
基金
上海市卫生计生委青年科研项目(20134Y040)
上海市卫生计生系统重要薄弱学科建设项目(2015ZB0203)
关键词
发育内皮基因1
细胞间黏附分子1
白细胞介素17
特异性过敏
Developmental endothelial locus-1
Intercellular cell adhesion molecule-1
Interleukin 17
Atopic allergy