邻苯二甲酸二异癸酯对小鼠学习记忆的影响

Effects of Di-iso-decyl phthalate on the learning-memory ability in mice

为研究增塑剂邻苯二甲酸二异癸酯(DIDP)对小鼠学习和记忆能力的影响,以白藜芦醇(Res)作为保护剂,将42只雄性昆明小鼠随机分为6组,每组7只,分别为:生理盐水组、1.5,15,150mg/(kg?d)DIDP组、20mg/(kg?d)Res组、150mg/(kg?d)DIDP+20mg/(kg?d)Res组.连续灌胃染毒9d,期间同时进行Morris水迷宫实验.第10d将小鼠处死,取出脑组织,检测活性氧簇(ROS)、谷胱甘肽(GSH)、丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHd G)、肿瘤坏死因子(TNF-α)、白细胞介素1β(IL-1β)的含量.并对小鼠海马体切片进行H&E染色,观察病理变化.结果显示,15,150mg/(kg?d)DIDP染毒可导致小鼠的学习和记忆能力显著低于对照组,同时可诱发脑组织产生氧化应激,并促进炎症因子释放,而Res能有效减弱脑组织氧化应激水平.由上可得,DIDP致小鼠学习和记忆能力下降可能与其引起的脑组织中海马体的氧化损伤有关,同时Res可能通过降低DIDP引起的氧化应激进而减轻其对小鼠造成的学习记忆损伤.

To elucidate the effect of di-iso-decyl phthalate （DIDP） on the learning and memory ability of mice, 42male Kunming mice were divided into 6groups randomly with 7mice in each group, including saline control group, the experimental groups of DIDP （1.5, 15, 150mg/（kg？d））, 20mg/（kg？d） Res control group, using as an anti-oxidant, 150mg/（kg？d） DIDP＋20mg/（kg？d） Res group. During the constant exposure for 9days, the Morris water maze was used to test the abilities of learning and memory of mice. Reactive oxygen species （ROS）, glutathione （GSH）, malonaldehyde （MDA）, 8-hydroxy-2deoxyguanosine （8-OHdG）, tumor necrosis factor alpha （TNF-α） and interleukin-1beta （IL-1β） levels in the brain of mice were measured after their sacrifice. H＆E-stained slices of hippocampus in brain tissue were applied to observe histopathological changes. The results indicated that the learning and memory ability of the 15、150mg/（kg？d） DIDP groups were significantly lower than the control group, while decreased significantly with the increase of DIDP concentrations. The 15, 150mg/（kg？d） DIDP could also induce oxidative stress in brain tissue and promote the release of inflammatory factors, while some of these effects were blocked by the administration of Res. Thus, it is concluded that DIDP-induced decrease of learning and memory ability in mice may be related to oxidative damage in brain tissue, while Res contributes to reduce the learning and memory damage in mice caused by DIDP effectively.