蝴蝶兰丛生芽组织培养研究进展

Advances in clusterd buds tissue culture of Phalaenopsis

蝴蝶兰市场前景广阔,采用组织培养快速繁殖种苗是规模化生产及推广应用的唯一手段,综述了蝴蝶兰丛生芽组织培养研究进展,旨在为蝴蝶兰种苗规模化生产提供依据。以花梗第2节或第3节为外植体,用0.1%氯化汞消毒8~15 min比次氯酸钠效果好;以1/2MS或花宝一号为基本培养基,使用6-BA或6-BA和NAA相搭配可诱导出丛生芽;以MS或花宝一号为基本培养基,6-BA或TDZ并配合NAA适宜的浓度,对丛生芽增殖效果明显;以低浓度的NAA或IBA,并配以椰子汁或香蕉汁、土豆汁等有机物对壮苗生根有较好的促进作用;活性炭能显著抑制褐化且对褐化的影响最大,转接周期对褐化的影响次之。

The Phalaenopsis market has broad prospects,the rapid breeding of seedlings by tissue culture is the only method for the mass production of Phalaenopsis. The clusterd buds tissue culture of Phalaenopsis was reviewed in order to provide a basis for the scale production. As the explant in section 2 or 3 of the pedicel,0.1% chlorinated mercury disinfected 8-15 min was better than sodium hypochlorite. The basic medium of 1/2 MS or hyponex No.1,and 6-BA or 6-BA and NAA could be used to induce clumps. 6-BA or TDZ and the appropriate concentration of NAA had a significant effect on the proliferation of clusterd buds,and the basic culture medium was either MS or hyponex No.1. The low concentration of NAA or IBA,and the organic matter such as coconut juice or banana juice,potato juice and so on had a good effect on the rooting. The activated carbon could inhibit browning and the influence of browning was first,and the influence of transition cycle on browning was secondary.