松墨天牛14-3-3ζ基因的鉴定及生物信息学分析

Identification and bioinformatics analysis of 14-3-3ζ gene from Monochamus alternatus

为探究松墨天牛(Monochamus alternatus)14-3-3ζ基因特性,通过构建cDNA文库,从松墨天牛cDNA文库中克隆到14-3-3ζ基因,命名为Ma14-3-3ζ,GenBank登录号为KT970073.1。克隆得到的Ma14-3-3ζcDNA全长为902 bp,其中开放阅读框为747 bp,编码248个氨基酸。推测的Ma14-3-3ζ蛋白等电点为4.73,分子量为28.16 ku。Ma14-3-3ζ的氨基酸序列与大黄粉虫(Tenebrio molitor)同源性最高、为98%,与褐飞虱(Nilaparvata lugens)等12种昆虫同源性在95%~90%之间。系统发育树表明松墨天牛与大黄粉虫在同一分枝上。

In order to elucidate the characteristics of Monochamus alternatus,the 14-3-3ζ gene from M.alternatus called Ma14-3-3ζ was cloned by using cDNA library construction,GenBank(KT970073.1). The cloned cDNA of Ma14-3-3ζ is 902 bp in length,which contains a 747 bp open reading frame( ORF) encoding 248 amino acids with a calculated molecular mass of 28.16 KD and an isoelectric point of 4.73. The results also indicate that Ma14-3-3ζ show the highest homology with Tenebrio molitor( 98% ),and has 95% -90% amino acid sequence homology with the 14-3-3ζ from 12 species of insects such as Nilaparvata lugens,M. alternatus and T. molitor on the same branch in the phylogenetic tree based on insect 14-3-3ζ analysis.