摘要
建立液相色谱-质谱联用仪测定植物源性食品中4种鼠药残留的方法。考察不同的流动相及净化方式对检测结果的影响。粮谷类试样经乙腈超声提取上机,果蔬类以乙腈提取后采用PSA+GCB管进行净化,LC/MS-MS进行检测。添标水平为0.002~0.050mg/kg时的回收率均达到70%以上,相对标准偏差为4.1%~9.5%,杀鼠醚和杀鼠灵的方法检出限为0.001mg/kg,其它2种鼠药的检出限为0.005mg/kg。该方法快速、简单、灵敏度高,符合GB/T 27404—2008对方法确认技术参数的要求。
To establish a LC/MS MS method for determination of 4 kinds of rodemieide residues in plant-origin food, the effects of differ ent mobile phase and purification on the recovery was studied. The grain samples were extraeted by acetontrile and then determined by LC/MS-MS, while the samples with fruit or vegetable matrix were purified with PSA＋ GCB after the extraction. LC/MS-MS was used for determinatkm. The recoveries were all above 70% at spiked levels of 0.002-0.050 mg/kg, and the relative standard deviation of the methods were 4.1%-9.5%. The I.OD of warfarin and coumatetralyl were 0.001 mg/kg while the other two reach 0.005 mg/kg which was in accordance with the requirement of technical parameters for analy- sis, with fast and simple determination and high sensitivity.
出处
《食品与机械》
CSCD
北大核心
2017年第11期72-75,共4页
Food and Machinery
基金
湖南省食品药品安全科技项目(编号:湘食药科R201608)
