百里香酚-壳聚糖复合膜及其在猪肉保鲜中的应用

The thymol-chitosan composite membrane and its application in pork preservation

研究百里香酚对单核增生李斯特氏菌的抑菌活性及抑菌机理,制作百里香酚-壳聚糖复合膜(CH/TO),研究复合膜的性质及其对猪肉的保鲜作用。结果表明,百里香酚对单核增生李斯特氏菌的最小抑菌浓度为12.5μg/mL;百里香酚能造成单核增生李斯特氏菌电导率增加,核酸和蛋白质大量渗出;随着复合膜中百里香酚浓度的不断增加(0.25%,0.50%,1.00%),膜的硬度和穿刺强度逐渐降低,百里香酚复合膜(CH/TO)具有一定抗氧化能力,温度和百里香酚浓度会影响复合膜对DPPH自由基的清除,25℃时1.00%百里香酚复合膜的清除率最高;百里香酚-壳聚糖复合膜能抑制猪肉中的微生物生长繁殖,降低菌落总数,延长猪肉的冷藏期。

The bacteriostatic activity and bacteriostatic mechanism of thymol to Listeria monocytogenes were studied and the thymol-chi- tosan composite membrane was prepared to study the properties of the composite membrane and its preservation effect on pork. The re sults showed that the minimum inhibitory concentration of thymol on Listeria monocytogenes was 12.5 μg/mL. The experimental study of conductivity, nucleic acid and protein leakage found that thymol caused by mononuelear proliferative Leptospira cell membrane dam age, so that bacterial cell inclusions caused by bacterial death. With the increase of thymol concentration （0.25%, 0.50%, 1.00%） in the thymol-chitosan composite membrane, the hardness and puncture strength of the membrane gradually decreased. The temperature and thymol concentration affected the removal of DPPH free radicals by composite membranes. At 25 ℃, the scavenging rate of 1.00% thy-mol-composite membrane was the highest at 25℃ ; the growth and reproduction of microbes in pork were reduced by the thymol- chitosan composite membrane, the total number of colonies decreased and the chilling period of pork was prolonged.