湘薯系列甘薯脱毒快繁技术研究（英文）

Study on Virus-free Culture and Rapid Propagation Technology of Xiangshu Series Varieties of Sweet Potato

[目的]甘薯病毒病对甘薯产量和品质的危害较大,是造成甘薯品种退化的直接原因,严重时会引起"绝收"。因此,加强甘薯病毒的检测和脱除,建立甘薯快繁技术方法,对保障甘薯的优良性状稳定遗传、预防甘薯病毒的扩大传播,促进甘薯产业的发展具有重要意义。[方法]该研究以湘薯15号和湘薯19号为研究材料,确定了适宜不同甘薯品种茎尖分生组织生长的脱毒培养方案,并建立了脱毒苗组培快繁技术。[结果]通过比较茎尖成苗率,确定湘薯15号的MS培养基最优植物激素添加方案为:6-BA 3.0 mg/L+NAA 1.0 mg/L。湘薯19号的MS培养基最优植物激素添加方案为:6-BA2.0 mg/L+NAA 0.67 mg/L。室内甘薯脱毒苗的繁殖系数为49 152,远远超过大田一年繁殖20 000株的速度。[结论]该研究立足于生产实际,结合茎尖分生组织脱毒技术,提出了集病毒检测、预警、脱除和繁育于一体的综合防控技术措施,对进一步提高我国优质甘薯的产量和质量,保证我国甘薯产业的健康发展具有重要战略意义。

[Objective] Sweet potato virus disease had a significant harm to the yield and quality of sweet potato, directly causing the degradation of sweet potato vari- eties and even the harvest failure. Therefore, the detection and removal of sweet potato virus and the establishment of rapid propagation method of sweet potato is of great significance to ensure the stable inheritance of excellent characters of sweet potato, prevent the spread of sweet potato virus and develop sweet potato industry. [Method] With Xiangshu series varieties of sweet potato, Xiangshu 15 and Xiangshu 19 as the research materials, a virus-free culture program was established for meristem tip apex tissue culture of different cultivars, and a rapid propagation method was developed for virus-free seedlings. [Result｜ On the basis of analysis on seedling emergence rate, the optimal addition scheme of plant hormones in the MS culture medium of Xiangshu 15 was 6-BA 3.0 mg/L ＋ NAA 1.0 mg/L, and the opti- mal plant hormone addition scheme for Xiangshu 19 was 6-BA 2.0 mg/L ＋ NAA 0.67 mg/L Under the developed rapid propagation system, the annual reproductive coefficient was up to 49 152, far higher than that （20 000） in field. IConclusionl Based on the actual production, combined with the meristem tip apex tissue culture, a comprehensive prevention and control measure was put forward, which included virus detection, early warning, removal and virus-free seedlings breeding, tt was of great strategic significance to improve the yield and quality of high-quality sweet potato and ensure the healthy development of sweet potato industry in China.