PP333与外源ABA对轮台白杏叶片组织结构的影响（英文）

Effects of PP333 and Exogenous ABA on Leaf Tissue Structure of Armeniaca vulgaris ‘Luntaibaixing'

[目的]探讨PP333和外源ABA对轮台白杏生长发育的影响,为轮台白杏栽培管理中的生长调控提供理论依据。[方法]以轮台白杏为试材,在新梢速长期喷施不同浓度的PP333和ABA,采用石蜡切片技术,测量叶片厚度、上表皮和下表皮厚度以及栅栏组织厚度和海绵组织厚度,计算栅海比、叶片组织结构紧密度(CTR)、叶片组织结构疏松度(SR),比较叶片组织结构的变化。[结果]PP333处理后,叶片厚度和栅栏组织厚度显著增加,其中1 000 mg/L处理增加效果最显著,同时栅海比和CTR值最大,而海绵组织厚度和SR值最小。ABA处理后的叶片厚度和栅栏组织厚度均有所增加,相比CK,60 mg/L处理增加最多。另外,60 mg/L处理的叶片栅海比和CTR值最大,海绵组织厚度和SR值最小。[结论]ABA和PP333处理后,叶片厚度和栅栏组织厚度均有所增加,同时也增大了栅海比和CTR值,而海绵组织厚度和SR值有所降低。

[Objective] This study aimed to investigate the effects of PP333 and ex- ogenous ABA on the growth and development of Armeniaca vulgaris ＇Luntaibaixing＇ so as to provide a theoretical basis for the growth regulation of Luntaibaixing in cultivation and management. [Method} Different concentrations of PP333 and ABA were sprayed to Luntaibaixing during the rapid growth period of fresh treetops. The technology of paraffin section was used to measure the thickness of leaf, upper epidermis, lower epidermis, palisade tissue and sponge tissue. The thickness ratio of palisade tissue to sponge tissue, tightness degree of leaf tissue structure （CTR） and loose degree of leaf tissue structure （SR） were calculated and the differences in leaf tissue structure were analyzed. [Result] The thickness of leaf and palisade tissue both increased significantly after treated by PP333. At the treatment concen- tration of 1 000 mg/L, the thickness ratio of palisade tissue to sponge tissue and the value of CTR were highest, while the thickness of sponge tissue and the value of SR were lowest. The thickness of leaf and palisade tissue also increased after treated by ABA, and it increased most greatly at the ABA concentration of 60 mg/L compared with CK. At the treatment concentration of 60 rag/L, the thickness ratio of palisade tissue to sponge tissue and the value of CTR were the highest, while the thickness of sponge tissue and the value of SR were the lowest. [Conclusion] ABA and PP333 treatment increased the leaf thickness, palisade tissue thickness, pal- isade tissue to sponge tissue thickness ratio and CRT value, but reduced the sponge tissue thickness and SR value of Luntaibaixing.