红麻微卫星DNA标记指纹图谱的构建

Construction of DNA Fingerprinting in Kenaf( Hibiscus cannabinus) using Microsatellite Markers

DNA指纹图谱对新品种选育、种质资源保存和管理具有重要的意义。然而,利用SSR标记构建红麻DNA指纹图谱的研究仍十分有限。在本研究中,利用课题组开发并筛选出的131对SSR引物,分析不同来源的96份红麻种质资源,包括红麻品种审定的区试对照品种福红952。结果表明,131对引物共扩增出375条带,平均每对引物扩增出2.6条带。以遗传相似系数0.614为切割线时,可以分为2个类群,52个为类群P1,44个为类群P2;以遗传相似系数0.710做切割线,可分为5个亚群。利用这131对引物标记所得的数据成功绘制了一份85个品种独特的指纹图谱,其中福红952可被HcEMS238引物特异识别。其他11份因存在遗传相似性高的现象,未被识别。上述结果为红麻品种的真实性鉴定及遗传多样性分析提供依据。

DNA fingerprinting is very important for the breeding of new varieties, germplasm resources conservation and management. However, DNA fingerprinting in kenaf using SSR markers is still very limited. In this study, the DNA fingerprint for 96 kenaf accessions, including the control variety Fuhong 952 ,were constructed on the basis of 131 pairs of SSR primers. The results showed that 131 pairs of primers amplified 375 bands,with the average of 2.6 bands per primer. When genetic similarity coefficient was 0. 614, all the kenaf accessions could be divided into two groups, P1 and P2. The group P1 included 52 accessions while P2 had 44 ones. When genetic similarity coeffi- cient was 0. 710, all the kenaf accessions could be divided into five subgroups. On the basis of 131 SSRs ,85 acces- sions had specific DNA fingerprints. Among them, HcEMS238 could differentiate Fuhong 952 from the others. The fingerprint of the other 11 accesssions failed to be constructed due to the presence of high genetic similarity. These results would be helpful in varieties authenticity identification and analysis of genetic diversity in kenaf.