氧化应激与实热“上火”的相关性研究

Study on Correlation between Oxidative Stress and Excess Heat “Shang huo”

目的:通过检测观察组血液中过氧化指标(MDA、ROS)和抗氧化指标(GSH、T-AOC、SOD)的变化,探讨氧化应激与实热"上火"的相关性。方法:分别收集临床实热"上火"和正常人群的样本,检测其血浆中MDA、GSH、SOD、T-AOC水平和外周血白细胞中ROS含量,进行比较。结果:与对照组比较,观察组血浆中MDA、GSH、SOD、T-AOC浓度水平均升高,差异有统计学意义(P<0.05),而白细胞内的ROS含量显著下降,差异有统计学意义(P<0.01)。结论:观察组过氧化指标与抗氧化指标均有显著变化,说明实热"上火"与氧化应激密切相关,观察组机体处于过氧化与抗氧化相互交争状态,但氧化与抗氧化水平尚且处于平衡阶段。

Objective： To study the correlation between oxidative stress and excess heat＂shanghuo＂through observing the changes of blood lipid peroxidation index（ MDA,ROS） and antioxidant indexes（ GSH,T-AOC,SOD） in the population of excess heat＂shanghuo＂. Methods： Excess heat＂shanghuo＂and normal population samples were collected,and the levels of MDA,GSH,SOD and T-AOC in plasma and ROS in peripheral blood leukocytes were detected and compared respectively. Results： The levels of MDA,GSH,SOD and T-AOC in the blood plasma of excess heat＂shanghuo＂were higher than those in the normal group（ P〈0. 05）,and the levels of ROS in leukocytes decreased significantly（ P〈0. 01）. Conclusion： The excess oxidation index（ MDA）and antioxidant index（ GSH,SOD,T-AOC） were all increased in excess heat＂shanghuo＂people,and the levels of ROS in leukocytes decreased significantly,indicating that excess heat ＂shanghuo＂is closely related to oxidative stress; the organism is in a level state of oxidation and antioxidation,but the oxidation and antioxidant levels are in balance.