健脾安肠丸对腹泻型肠易激综合征结肠组织SP、VIP、CGRP的影响

Effects of Jianpi Anchang Pill on SP,VIP and CGRP in the Colon Tissue of Diarrhea-predominate Pattern Irritable Bowel Syndrome

目的:研究健脾安肠丸对腹泻型肠易激综合征(IBS-D)结肠组织SP、VIP的影响。方法:采用大黄灌胃与束缚配合的方法制备IBS-D大鼠。将60只成年SD大鼠随机分为4组:正常组、模型组、健脾安肠丸大剂量组、健脾安肠丸小剂量组和匹维溴铵组。采用ELISA法检测大鼠结肠组织中P物质(SP)、血管活性肠肽(VIP)和降钙素基因相关肽(CGRP)的含量。结果:模型组大鼠大便形状的评分值较正常组明显增高(P<0.01),而健脾安肠丸大剂量组和匹维溴铵组较模型组分值显著下降(P<0.01,P<0.05);大剂量、小剂量健脾安肠丸和匹维溴铵均能减少模型大鼠结肠SP、VIP含量,提高CGRP含量。结论:健脾安肠丸能有效地缓解腹泻型肠易激综合征,其疗效可能与减少SP、VIP和增加CGRP的释放有关。

Objective： To study the effects of Jianpi Anchang pill on SP and VIP in the colon tissue of Diarrhea-predominate Pattern Irritable Bowel Syndrome（ IBS-D）. Methods： The IBS-D rat model was established by gavage of Radix et Rhizoma Rhei and binding limbs. A total of 60 adult SD rats were randomly divided into 4 groups： normal group,model group,Jianpi Anchang pill high dose group and Jianpi Anchang pill low dose group and Pinaverium Bromide group. The contents of SP,VIP and CGRP in the rat colon tissue were determined by ELISA. Results： In the model group,fecal shape score was much higher than that of normal group（ P〈0. 01）,while the score values of Jianpi Anchang pill high dose group and Pinaverium Bromide group were lower than model group（ P〈0. 01,P〈0. 05）; High,low dose of Jianpi Anchang pill and Pinaverium Bromide could decrease the contents of SP ad VIP and enhance the contents of CGRP in colon tissue of the model rats. Conclusion： Jianpi Anchang Pill can effectively ease IBS-D,and its effects may be related to reduce the SP and VIP and increase CGRP release.