基于指纹图谱技术分析四神丸中吴茱萸的有效成分

Analysis of Effective Components of Fructus Evodiae in Shishen Pill Based on Fingerprint Technology

目的:基于指纹图谱技术分析探讨四神丸中吴茱萸的有效成分,阐释中药合理配伍的科学内涵。方法:精密量取10μL的样品进样量或对照品溶液,采用250 mm×4.6 mm,5μm的色谱柱,选择流动相的条件是在乙腈、甲醇和水中以1m L/min的流速进行梯度洗脱,柱温为30℃,检测波长为225 nm,根据高效液相(HPLC)指纹图谱的梯度洗脱程序进行试验,并在HP HPLC(3D)化学工作站上进行指纹图谱分析四神丸中吴茱萸的有效成分。结果:四神丸HPLC指纹图谱检测精密度、重复性和稳定性均良好。不同批次四神丸中的吴茱萸的有效成分是吴茱萸碱和吴茱萸次碱,不同厂家四神丸中吴茱萸的有效含量有明显差异有统计学意义(P<0.05),以同仁堂四神丸中吴茱萸的有效成分含量为最多,其次为仲景牌四神丸。结论:基于指纹图谱技术用高效液相色谱法分析四神丸中吴茱萸的有效成分测定,发现其有效成分主要是吴茱萸碱和吴茱萸次碱,进而对该药物进行质量控制。

Objective： To explore the effective constituents of Fructus Evodiae in Shishen Pill with fingerprint technology based on the scientific connotation of the reasonable compatibility of Chinese medicine. Methods： The precise amount of 10 μL sample or standard solution was obtained. 250 mm × 4. 6 mm,5 μm chromatographic column was used,and mobile phase condition was in acetonitrile,methanol and water at a flow rate of 1 m L/min by gradient elution. The column temperature was 30 ℃,and the detection wave length was 225 nm. According to HPLC（ high performance liquid chromatography）,fingerprint gradient elution program was tested,and effective constituents of Fructus Evodiae in Shishen Pill was analyzed with fingerprint based on HP HPLC（ 3 D）chemical workstation. Results： The precision,repeatability and stability of Sishen Pill HPLC fingerprint detection were good. The effective components of Fructus Evodiae in Shishen Pill with different batches were evodiamine and rutaecarpin. There was significant difference in the effective contents of Fructus Evodiae in Shishen Pill of different manufacturers（ P〈0. 05）. The content of effective components of Fructus Evodiae in Shishen Pill in Tongrentang was the most,followed by Zhongjing Brand. Conclusion： The technology of fingerprint by HPLC analysis of effective constituents of Fructus Evodiae in Shishen Pill based assay found that the main active component is evodiamine and rutaecarpine,and control the quality of the drug.