黄芪白术配伍前后黄芪化学成分分析研究

Study on Chemical Constituents of Radix Astragali seu Hedysari Before and After Compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae

目的:基于HPLC分析黄芪白术配伍前后黄芪化学成分分析研究,阐释中药合理配伍的科学内涵。方法:采用高效液相(HPLC)指纹图谱分析进行线性关系分析以及加样回收率试验考察HPLC的严谨性。HPLC指纹图谱:取黄芪甲苷对照品溶液、缺黄芪阴性对照溶液、黄芪供试品溶液和黄芪白术配伍供试品溶液进行HPLC指纹图谱分析,同时取3个产地黄芪白术分析黄芪化学成分含量。结果:1)在线性关系考察在1.024~10.24μg范围内线性关系良好。2)加样回收率试验结果中平均回收率为99.60%,RSD为2.0%,显示加样回收率试验良好,HPLC严谨性高。3)HPLC指纹图谱分析显示,对照品溶液、黄芪供试品溶液、黄芪白术供试品溶液和缺黄芪阴性对照溶液中,缺黄芪阴性对照溶液无干扰,而黄芪甲苷与其他成分的基线分离良好。4)3个产地均显示黄芪白术配伍后黄芪的化学成分黄芪甲苷的含量有所增加,其中以内蒙古产地的黄芪白术配伍中黄芪甲苷的含量最高。结论:黄芪白术配伍前后黄芪的主要化学成分为黄芪甲苷,其中以内蒙古提供的药材含量最高。

Objective： To analyze and study the chemical constituents of Radix Astragali seu Hedysari before and after compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae based on HPLC,and to explain the scientific connotation of the rational compatibility of Chinese herbal medicine. Methods： The linear relationship analysis was conducted by HPLC and the sample recovery test was used to investigate the preciseness of HPLC. HPLC fingerprint： astragaloside reference solution,negative reference solution lack of Radix Astragali seu Hedysari,Radix Astragali seu Hedysari test solution and the compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae test solution for HPLC fingerprint analysis,and chemical constituents of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae from 3 areas were analyzed. Results： 1） The linear relationship was good in the range of 1. 024 ~ 10. 24 μg,and with 2. 2 sample recovery test results. 2） The average recovery rate was 99. 60%,and RSD was 2%,which showed that the sample recovery rate was good,and the preciseness of HPLC was high. 3） HPLC fingerprint analysis showed that in reference solution,negative reference solution lack of Radix Astragali seu Hedysari,Radix Astragali seu Hedysari test solution and the compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae test solution,no interference in negative reference solution lack of Radix Astragali seu Hedysari and the baseline separation of astragaloside and other components were good. 4） Analysis in 3 ares showed that the content of astragaloside increased with the compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae,among which that content was the highest from Inner Mongolia. Conclusion： The main chemical constituents of Radix Astragali seu Hedysari after the compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae,among which the content in Inner Mongolia is the highest.