孕鼠七氟醚麻醉或手术对子代小鼠社会交往行为的影响

Effects of maternal exposure to sevoflurane for anesthesia or surgery on social interaction behaviors in offspring mice

目的 评价孕鼠七氟醚麻醉或手术对子代小鼠社会交往行为的影响.方法 选取妊娠18 d的C57BL∕6小鼠15只,采用随机数字表法分为3组（n=5）：对照组（C组）吸入100%氧气2 h;七氟醚麻醉组（Sevo组）吸入3%七氟醚＋氧气2 h;手术组（Surg组）吸入3%七氟醚＋氧气2 h,并实施腹腔探查手术.在子代小鼠幼年期（即出生后第30天）和成年早期（即出生后第60天）,采用三室社会交往模型测试子代小鼠的社会交往行为,包括社会交往倾向和社会新奇偏好.结果 第1测试阶段：3组子代小鼠主动探索左右两侧房间的时间、嗅探左右侧空铁笼的时间比较差异无统计学意义（P〉0.05）.第2测试阶段：与空铁笼比较,3组子代小鼠在幼年期和成年早期探索陌生鼠-1铁笼所在房间的时间、嗅探陌生鼠-1铁笼的时间延长（P〈0.05）.第3测试阶段：与陌生鼠-1比较,C组子代小鼠幼年期探索陌生鼠-2房间的时间、幼年期和成年早期嗅探陌生鼠-2铁笼的时间延长,Sevo组子代小鼠成年早期探索陌生鼠-2房间的时间、嗅探陌生鼠-2铁笼的时间延长,Surg组成年早期嗅探陌生鼠-2铁笼的时间延长（P〈0.05）.结论 孕鼠接受七氟醚麻醉或七氟醚麻醉下腹腔手术可导致子代小鼠社会交往缺陷,表现为社会新奇偏好减弱,且主要表现在幼年期.

 Objective To evaluate the effects of maternal exposure to sevoflurane for anesthesia or surgery on social interaction behaviors in offspring mice. Methods Fifteen pregnant C57BL∕6 mice on ges-tational day 18 were assigned into 3 groups（n=5 each）using a random number table： control group （group C）, sevoflurane anesthesia group（group Sevo）and surgery group（group Surg）. Group C inhaled 100% oxygen for 2 h in group C. Group Sevo inhaled 3% sevoflurane in oxygen for 2 h. In group Surg, 3%sevoflurane in oxygen was inhaled for 2 h and exploratory laparotomy was performed. At juvenile age（on 30th day after births）and in early adulthood（on 60th day after births）, the offspring mice underwent so-cial interaction tests using the three-chambered sociability paradigm, including sociability and preference for social novelty. Results During 1st testing phase： there was no significant difference in time spent in ex-ploring the chamber on the left and right sides or in sniffing directed at the empty wire cage on the left and right sides among the three groups（P〉0.05）. During 2nd testing phase： time spent in exploring the cham-ber containing stranger-1 mouse in the wire cage and in sniffing directed at the wire cage containing stranger-1 mouse was significantly prolonged at juvenile age and in early adulthood in three groups when compared with the empty wire cage（P〈0.05）. During 3rd testing phase： compared with stranger-1 mouse, time spent in exploring the chamber containing stranger-2 mouse at juvenile age and in sniffing directed at the wire cage containing stranger-2 mouse at juvenile age and in early adulthood was significantly prolonged in group C, time spent in exploring the chamber containing stranger-2 mouse and in sniffing directed at the wire cage containing stranger-2 mouse was significantly prolonged in early adulthood in group Sevo, and time spent in sniffing directed at the wire cage containing stranger-2 mouse was significantly prolonged in early adulthood in group Surg（P〈0.05）. Conclusion Maternal exposure to sevoflurane for anesthesia or surgery induces social interaction deficits in the offspring mice in which lower levels of preference for social novelty are detected, especially at juvenile age.