miR-21及PDCD4在非小细胞肺癌的表达及其临床意义

The expressions of miR-21 and PDCD4 in non-small-cell lung cancer patients and its clinical significances

目的:探讨人微小核糖核酸21(miR-21)与程序性细胞死亡因子4(PDCD4)在非小细胞肺癌中的表达与临床病理特征之间的关系。方法:采用荧光定量PCR(qPCR)法检测53例非小细胞肺癌组织及其配对癌旁组织中miR-21与PDCD4表达情况。结果:非小细胞肺癌组织中miR-21表达明显高于配对癌旁肺组织(P<0.01),非小细胞肺癌组织中PDCD4表达明显低于配对癌旁肺组织(P<0.01)。miR-21 mRNA的高表达与非小细胞肺癌淋巴结移、临床分期相关(P<0.05);PDCD4mRNA的低表达与非小细胞肺癌淋巴结移、临床分期相关(P<0.05);miR-21与PDCD4在非小细胞肺癌组织中的表达呈负相关关系(r=-0.89,P<0.01)。结论:miR-21高表达与PDCD4的低表达与非小细胞肺癌的发生、转移密切相关。

Objective： To investigate the expressions of microscopic RNA-21（miR-21）and programmed cell death factor 4（PDCD4）in non-small-cell lung cancer（NSCLC）patients and its clinical significances. Methods： Lung cancer tissues and pericancer tissues were collected from 53 NSCLC patients. The expressions of miR-21 and PDCD4 were detected by fluorescent quantitative PCR（qPCR）. Results： The expression of miR-21 in NSCLC tissues was significantly higher than that in the adjacent tissues,while the PDCD4 expression was lower（P〈0. 01）. Overexpression of miR-21 in NSCLC was associated with advanced clinical stage and lymph node metastasis,but low expression of PDCD4 was associated with lymph node metastasis and advanced TNM stage（P〈0. 05）. There was a negative correlation between the expressions of miR-21 and PDCD4 in NSCLC patients. Conclusion： Higher expression of miR-21 and lower expression of PDCD4 were closely related to the development of NSCLC.