COG复合体亚基COG2和COG3之间相互作用的研究

Identification of the interactions between subunits COG2 and COG3 in COG complex

真核细胞内的囊泡运输过程分为四个环节:囊泡的出芽、运输、栓系和融合.这其中的囊泡栓系过程是由栓系因子所介导的.位于高尔基体上的一类栓系因子COG复合体在介导高尔基体内部的囊泡逆向运输过程中发挥着重要的作用.COG复合体由八个亚基组成,本文首先通过基因克隆的方法,克隆获得两个COG亚基COG2和COG3的基因.随后的工作中,通过两种蛋白质之间互作的检测方法,进一步地证明了COG2和COG3两个亚基之间存在着相互作用.

Each vesicle transport reaction can be divided into four essential steps that include vesicle budding,transport,tethering and fusion. The third step tethering in vesicle-mediated membrane traffic is mediated by tethering factors. One of the tethering factors,the COG complex is essential for vesicle transport within the Golgi apparatus. Architecturally,the eight subunits that make up the COG complex can be divided into two subassemblies,lobe A( Cog1,Cog2,Cog3,and Cog4) and lobe B( Cog5,Cog6,Cog7,and Cog8). To test the interactions between subunits COG2 and COG3,we began with the cloning of COG2 and COG3. Subsequently,we prove protein-protein interactions between COG2 and COG3.