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促红细胞生成素对缺氧缺血性脑损伤新生Wistar大鼠海马GFAP和BrdU表达的影响 被引量:8

Effects of erythropoietin on the expression of GFAP and Brd U in hippocampus of neonatal Wistar rats with hypoxic-ischemic brain damage
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摘要 目的探讨外源性促红细胞生长素(EPO)对新生Wistar大鼠缺氧缺血性脑损伤(HIBD)后海马CA1区胶质纤维酸性蛋白(GFAP)和海马DG区5-溴-2-脱氧尿嘧啶核苷(BrdU)表达的影响。方法48只7日龄新生Wistar大鼠随机分为HIBD模型组、EPO实验组,另设假手术组24只;通过结扎右侧颈总动脉、并吸入8%O2加92%N2低氧混合气体2h制作HIBD模型;免疫组织化学法检测并比较三组新生鼠制模术后14d、21d、28d海马CA1区GFAP表达、以及DG区BrdU阳性细胞数的变化。结果术后14d、21d,三组新生Wistar大鼠海马CA1区GFAP以及DG区BrdU阳性细胞数的差异均有统计学意义(P<0.01),均以EPO实验组最多,HIBD模型组其次,假手术组最少;术后28d,三组间GFAP表达以及DG区BrdU阳性细胞数比较均无统计学意义(P>0.05)。各组大鼠不同时间(术后14d、21d、28d)之间比较,CA1区GFAP以及DG区BrdU阳性细胞表达的差异均有统计学意义(P<0.01),均以术后14d表达最多,21d表达次之,28d表达最少。结论早期给予外源性EPO治疗可促进新生鼠HIBD后海马CA1区GFAP表达增多,DG区BrdU阳性细胞增加,表明EPO可促进受损神经细胞增殖、再生;EPO在新生鼠缺氧缺血性脑损伤中发挥神经保护作用。 ObjectivesTo explore the effect of exogenous erythropoietin (EPO) on the expression of glial fbrillary acidic protein (GFAP) in hippocampal CA1 region and 5- bromide -2- uracil (BrdU) in hippocampal DG region in neonatal Wistar rats with hypoxic-ischemic brain damage (HIBD). MethodsForty-eight Wistar rats aged 7 days were randomly divided into HIBD model group and EPO experimental group, and another 24 rats as sham operated group. The HIBD model was established by ligating the right common carotid artery and inhaling hypoxia gas mixture (8% O2 and 92% N2) for 2 h. The expression of GFAP in hippocampal CA1 region and the number of BrdU positive cells in the hippocampus were detected by immunohistochemical method on at 14 d, 21 d, and 28 d after operation and compared among three groups. ResultsOn 14 d and 21 d after operation, the expression of GFAP in CA1 region and the number of BrdU positive cells were statistically different among three groups (P〈0.01) with EPO experimental group having the highest, HIBD model group having the second highest and sham operation group having the lowest in both, . On 28 d after operation, there was no difference in the expression of GFAP and the number of BrdU positive cells in the DG among three groups (P〉0.05). At different time point (14 d, 21 d, 28 d) in every group, the expression of GFAP in CA1 region and the number of BrdU positive cells in DG region were all statistically different (P〈0.01), all with the highest on 14 d after operation, second highest on 21 d, and the lowest on 28 d. ConclusionsEarly administration of exogenous EPO can promote the expression of GFAP in hippocampal CA1 region and increase the number of BrdU positive cells in DG region, which indicates that EPO can promote the proliferation and regeneration of damaged neurons. EPO had neuroprotective effect on neonatal rats with hypoxic-ischemic brain damage.
出处 《临床儿科杂志》 CAS CSCD 北大核心 2018年第1期65-68,共4页 Journal of Clinical Pediatrics
基金 贵州省科技厅社发攻关项目(黔科合SY[2009]3007号) 遵义市红花岗区科技局社合项目(遵红科社字[2009]19号)
关键词 促红细胞生成素 5-溴-2-脱氧尿嘧啶核苷 胶质纤维酸性蛋白 缺氧缺血性脑损伤 新生鼠 erythropoietin 5-bromo-2-deoxyuridine glial fbrillary acidic protein hypoxic ischemic brain damage neonatal rats
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