艾灸对新生小鼠脑卒中后抑郁模型体质量和行为学的影响

Effects of Moxibustion on Body Weight and Behavioral Performance in Neonatal Post-stroke Depression Mice

目的:观察艾灸对新生小鼠脑卒中后抑郁模型体质量和行为学的影响,观察艾灸治疗新生小鼠脑卒中后抑郁的效果。方法:ICR小鼠,随机分为3组(假手术组、脑卒中后抑郁模型组和艾灸组)。采用复合刺激制备脑卒中后抑郁小鼠模型,即于出生后7d给予新生小鼠脑缺血缺氧刺激,并于术后3w始给予21d慢性不可预见性温和应激。采用Open-Field测试小鼠的水平活动及垂直活动得分,蔗糖水摄入量测试小鼠糖水消耗百分比,以及Morris水迷宫测试小鼠的学习记忆力。结果:模型组小鼠体质量减轻,水平活动和直立活动得分均减少、糖水消耗百分比降低以及逃避潜伏期延长;而与模型组相比,艾灸组小鼠体质量增加,水平活动和直立活动得分增多、糖水消耗百分比也升高,而逃避潜伏期缩短。结论:艾灸对新生小鼠脑卒中后抑郁症状具有改善作用。

Objective： To observe the effect of moxibustion on body weight and behavioral results in post-stroke depression mice. Methods： ICR pups were randomly divided into 3 groups（ sham group,post-stroke depression（ PSD） model group,moxibustion group）.The PSD model was induced by combined stimuli,i. e. brain ischemia and hypoxia at 7 d postnatal,then 21 d of chronic unexpected mild stimulus（ CUMS） since 28 d postnatal. Open-Field was used to test crossing and rearing,sucrose consumption test was used to calculate the percentage of sucrose intake,Morris water maze was employed to evaluate learning and memory abilities. Results： Compared to mice in the sham group,mice in the PSD group showed that body weight was lowered,crossing scores and rearing scores were decreased,the percentage of sucrose intake was dropped and the escape period was lengthened. Compared to mice in the model group,mice in the moxibustion group showed that body weight was gained,crossing scores and rearing scores were increased,the percentage of sucrose intake was risen and the escape period was shortened. Conclusion： Moxibustion improves the symptoms of post-stroke depression.