生物过程代谢组学与代谢流测定

Determination of metabolomics and metabolic fluxes in bioprocesses

整合的代谢组学和13C代谢流数据能充分反映细胞的代谢状态,对代谢组学和代谢流的测定技术已成为工业生物过程研究的重要手段,并能为工业生物过程优化和高产菌株理性设计提供重要帮助。阐述了代谢组学和代谢流测定的完整流程,包括实验方法、数据处理方法和软件工具,并综述了其在代谢途径鉴定、代谢机制解析和代谢工程等领域的应用进展。

The integration of metabolomics and 13 C metabolic fluxes provide an adequate description of cell metabolic state. The determination of metabolomics and metabolic fluxes is crucial and helpful for the optimization of industrial bioprocesses and rational design of high production strains. This paper illustrates the entire workflows for determining metabolomics and metabolic fluxes, including experiment methods, data processing methods and software tools. The application of metabolomics and metabolic fluxes in the fi elds of metabolic pathway identifi cation, metabolic mechanism elucidation and metabolic engineering is also summarised.