家养双峰驼SRY基因的序列分析与拷贝数研究

Copy Number and Sequence Analysis of SRY Gene in Chinese Domestic Bactrian Camel

为探究中国家养双峰驼SRY基因的序列以及群体间拷贝数变异,对7个中国家养双峰驼群体共94个个体的SRY基因部分序列进行测序,分析SRY基因序列与其他物种的相似性;并且以CYP2A基因为内参基因,运用实时荧光定量PCR技术检测SRY基因的拷贝数。结果表明,中国家养双峰驼与人、马、猪、羊及普通牛的SRY基因相似度分别为67.7%~68.1%、68.5%~69.4%、73.8%~74.4%、73.8%~74.8%、74.1%~74.8%。在家养双峰驼SRY基因的多个拷贝中,除1个拷贝外,其余拷贝的HMG序列与羊驼、人、马、猪、绵羊及普通牛的HMG序列相比,相似度分别为97.3%、84.2%、85.6%、86.3%、87.0%、85.6%。SRY基因在中国家养双峰驼Y染色体上以多拷贝形式存在,变幅为1~9个拷贝。表明中国家养双峰驼SRY基因的HMG序列是高度保守的,而且该基因属于多拷贝基因。

The study aims to explore SRYgene sequence of Chinese domestic bactrian camel and the copy number variation of SRY among populations.Partial sequence of SRYgene of 94 samples from seven populations were sequenced to analyze the similarity among species.Additionally,CYP2A gene as a reference gene,SRYgene copy number was detected by real-time PCR.The similarity of SRY gene between Chinese bactrian camel and human,horse,pig,sheep as well as bovine was 67.7%-68.1%,68.5%-69.4%,73.8%-74.4%,73.8%-74.8% and 74.1%-74.8%,respectively.Except for one copy,the similarity between HMG of the rest copy of Chinese bactrian camel and HMG of alpaca,human,horse,pig,sheep as well as bovine was 97.3%,84.2%,85.6%,86.3%,87.0% and 85.6%,respectively.The SRYgene was a multicopy gene in Chinese domestic bactrian camel and copy number variation ranged from 1 to 9.HMG sequences of Chinese domestic bactrian camel were highly conserved.The SRYgene was a multicopy gene in Chinese domestic bactrian camel.