SSR标记技术鉴定‘新食葵7号’品种纯度的研究

Purity Identification of Sunflower Hybrid‘Xinshikui 7'Using SSR Markers Technique

以‘新食葵7号’及其双亲的DNA为模板,对100对SSR引物进行筛选,以期为生产应用提供准确、便捷的杂交种种子纯度鉴定方法。SSR多态引物标记521在‘新食葵7号’母本产生特异条带大小为482bp,父本为447bp;标记563在母本上特异条带大小为352bp,父本为384bp,分子鉴定的纯度和田间鉴定纯度基本一致,通过SSR引物筛选,得到可以区分父本、母本和杂交种的标记引物521和563,这2个引物标记可有效鉴定‘新食葵7号’杂交种种子的纯度。

SSR molecular marker technique was used to provide accurate,convenient method for the identification of the purity of sunflower hybrid seeds in production and processing.With the DNA of‘Xinshikui 7＇and its parents as template,100 pairs of SSR molecular markers were screened after DNA extraction,PCR amplification and electrophoresis production.The result showed that the SSR polymorphic primer marker 521 produced a specific band of 482 bp in the female parent,and a specific band of 447 bp in the male parent;the primer marker 563 produced a specific band of 352 bp in the female parent,and a specific band of 384 bp in the male parent.The indoor molecular purity identification were consistent with field purity identification.The primer marker 521 and 563 could be used to distinguish the male parent,female parent and hybrid of ‘Xinshikui 7＇,and both of the 2 primer markers can effectively identify the purities of the hybrid seeds of‘Xinshikui 7＇,as well as the authenticity of the seeds.The proposed method was simple,fast,and accurate to operate with the advantages of high reproducibility,and it had become the major method in the identification of sunflower varieties.