‘库尔勒香梨’幼果内源激素分布差异与果实萼片脱落的关系

The Relationship between Endogenous Hormones Distribution in Fruitlets and Calyx Shedding of‘Korla Fragrant Pear'

以‘库尔勒香梨’‘鸭梨’‘锦丰梨’为试验材料,探究‘库尔勒香梨’幼果不同部位内源激素分布差异与果实萼片脱落之间的关系。采用酶联免疫吸附测定法(ELISA)测定幼果萼端、中部和下部的IAA、ZR、GA3和ABA质量分数,进行差异显著性分析。结果表明,‘库尔勒香梨’脱萼幼果、‘鸭梨’幼果与‘库尔勒香梨’宿萼幼果、‘锦丰梨’幼果萼端IAA、ZR质量分数不存在差异性,而幼果下部IAA和ZR质量分数存在极显著性差异,‘库尔勒香梨’脱萼幼果和宿萼幼果下部ABA和GA3质量分数存在极显著性差异。‘库尔勒香梨’脱萼幼果和‘鸭梨’幼果中的IAA、GA3主要分布在萼端,ZR主要分布在中部,ABA主要分布在萼端和下部;宿萼幼果和‘锦丰梨’幼果中的IAA、ZR和GA3主要分布在幼果中部,ABA主要分布在幼果下部。在‘库尔勒香梨’萼片脱落临界期,脱宿萼幼果不同部位内源激素质量分数存在显著差异性,幼果下部IAA、ZR质量分数高有利于萼片宿存,幼萼片端GA3和ABA质量分数高可能有利于萼片脱落。

In this research,Korla fragrant pear,‘Yali pear＇and ‘Jinfeng pear＇were used as experimental materials to explore the relationship between fruit calyx shedding and endogenous hormone mass fractions in different parts of‘Korla fragrant pear＇fruitlets.Mass fractions of IAA,ZR,GA3 and ABA in three parts of‘Korla fragrant pear＇,‘Yali pear＇and‘Jinfeng pear＇fruitlets were determined using Enzyme-linked Immunosorbent Assays（ELISA）method,and the significant differences of the mass fractions were analyzed as well.The results showed that,there were no differences in the mass fractions of IAA and ZR in the calyx ends of‘Korla fragrant pear＇,‘Yali pear＇and ‘Jinfeng pear＇fruitlets,but there were significant differences in the mass fractions of IAA and ZR in the lower parts of their fruitlets,and remarkable differences existed in the mass fraction s of ABA and GA3 in the lower parts of‘Korla fragrant pear＇shedding calyx fruitlets and pesistant calyx fruitlets;besides,in‘Korla fragrant pear＇shedding calyx fruitlets and ‘Yali pear＇fruitlets,IAA and GA3 were mainly distributed in the calyx ends,ZR in the middle parts and ABA in the calyx ends as well as the lower parts,while in‘Korla fragrant pear＇persistant calyx fruitlets and ‘Jinfeng pear＇fruitlets,IAA,ZR,and GA3 mostly existed in the middle parts and ABA in the lower parts.Thus,it is concluded that significant differences exist in IAA,GA3,ZR and ABA mass fractions in ‘Korla fragrant pear＇fruitlets during the critical period of calyx shedding,high mass fractions of IAA and ZR in lower parts of fruitlets are conducive to the calyx persistence,and high accumulations of GA3 and ABA in the calyx ends are helpful to the calyx shedding.