流式细胞术的发展与展望

The advancement and perspective of flow cytometry

流式细胞术是一种基于单颗粒的高通量、高内涵检测的技术被广泛应用于生命科学和临床诊断，诞生至今已有52年的历史。近年来，流式细胞仪在液路、光路（包括新型染料）和信号转化、数据采集、计算等方面发展迅速，而且和质谱、微流控、图像处理和拉曼光谱等技术结合衍生出新型的流式细胞仪，为个性化医疗背景下血液学、免疫学、肿瘤学等提供单细胞水平的更快、更精、更广的组学信息。流式细胞术的标准化和质控管理的不断完善为流式细胞术的临床应用提供了坚实的保障。

Flow Cytometry acting as a popular technology based on single cell with high throughput and high content in all-round life science fields besides clinical diagnosis, has been launched for more than 52 years, With the advancement of fluidic path, photic path（including novel labeling dye） together with signal collection, transformation and algorithm et al. Particularly, traditional flow cytometry combined with mass spectrometry, microfluidic technology, image principle, Raman spectrometry and so on, resulted in flow cytometry being applied in hematology, immunology, oncology with an enhanced performance. Meanwhile, the improvement of standardization and quality control will boost the flow cytometry in clinical application. （Chin J Lab Med, 2018, 41： 20-23）