中链甘油三酯抗疲劳作用及其机制研究

Anti-fatigue effect of medium chain triglycerides and its mechanism

研究中链甘油三酯(MCT)的抗疲劳作用及其相关的机制。将KM雄性小鼠100只随机分为5组,每组20只,每组再随机分成2个小组,每小组10只,分别为安静对照组(CC_1和CC_2)、游泳对照组(SC_1和SC_2)、低剂量组(SL_1和SL_2)、中剂量组(SM_1和SM_2)、高剂量组(SH_1和SH_2)。1小组SC_1、SL_1、SM_1和SH_1在灌胃给药30 d后用于无负重游泳60 min实验,测定各生化指标;2小组SC_2、SL_2、SM_2和SH_2小鼠在灌胃给药30 d后用于负重游泳力竭实验,测定负重游泳力竭时间。结果表明:与游泳对照组相比,MCT可以显著延长小鼠负重游泳力竭时间,降低运动后血清尿素氮含量和血清乳酸含量,减少血糖和肝糖原消耗,提高肝脏超氧化物歧化酶活性、谷胱甘肽含量和游离脂肪酸含量,降低肝脏丙二醛含量,提高血清乳酸脱氢酶活性。因此,MCT具有抗疲劳的作用,且以低剂量组效果最明显。

The anti - fatigue effect of medium chain triglycerides （MCT） and related mechanism were studied. 100 healthy male KM mice were randomly divided into five groups：quiet control group （CC1and CC2 ）, swimming control group （SC1 and SC2 ）, low dose group （SLl and SL2 ）, middle dose group （ SM1 and SM2 ） and high dose group （ SH1 and SH2 ） , each group had 20 mice, and then each group was randomly divided into two groups, each group had 10 mice. The first group of mice SC1 , SL1 , SM1 and SH- would be swimming without load for 60 rain after the gastric perfusion of MCT for 30 d, and then the biochemical indicators were measured. The second group of mice SC2 ,SL2, SM2and SH2would be exhausted burden swimming after the gastric perfusion of MCT for 30 d, and then the burden swimming exhaus- tive time was measured. The results showed that compared with swimming control group, MCT could sig- nificantly prolong the burden swimming exhaustive time, reduce the serum urea nitrogen and serum lactic acid contents, reduce blood glucose and liver glycogen consumption, improve liver SOD activity, GSH content and FFA content, reduce the content of liver MDA and increase serum LDH activity. So MCT had anti -fatigue effect, and the effect was the most obvious in low dose group.